PMID:8360165

Citation	Pei, Z and Blaser, MJ (1993) PEB1, the major cell-binding factor of Campylobacter jejuni, is a homolog of the binding component in gram-negative nutrient transport systems. J. Biol. Chem. 268:18717-25
Abstract	The protein PEB1 (28 kDa) is a common antigen and a major cell adherence molecule of Campylobacter jejuni and Campylobacter coli. We created a bank of chromosomal DNA fragments of C. jejuni strain 81-176 using lambda gt11. Screening this bank in Escherichia coli Y1090 cells with antibody raised against purified PEB1 enabled us to isolate and to purify a clone with a 2.6-kilobase insert expressing an immunoreactive protein of 28 kDa. DNA sequencing revealed that the insert contains three complete and two partial open reading frames (ORFs), designated 5' to 3' as ORFs A-E. The peb1A gene (ORF D) contains 780 bases encoding a 259-residue polypeptide having a calculated molecular mass of 28,181 Da. The peptide sequence starting at residue 27 matches that determined from aminoterminal sequencing of mature PEB1 from C. jejuni. The first 26 residues contain typical signal peptidase I and II cleavage sites. The deduced amino acid composition and pI of the recombinant mature protein are similar to those determined for purified PEB1. Gene bank searches indicated significant overall homology of peb1A and ORF C with operons for amino acid transport systems in other Gram-negative organisms. peb1A is homologous to the binding components of systems such as glnH (27.8%) and hisJ (28.9%), whereas ORF C has nearly 50% identity to glnQ and hisP. Thus, PEB1 could be involved both in binding to intestinal cells and in amino acid transport.
Links	PubMed
Keywords	Amino Acid Sequence; Amino Acids/analysis; Amino Acids/metabolism; Antigens, Bacterial; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacteriophage lambda/genetics; Base Sequence; Biological Transport; Campylobacter jejuni/chemistry; Campylobacter jejuni/genetics; Cell Adhesion Molecules/chemistry; Cell Adhesion Molecules/genetics; Cloning, Molecular; Codon; DNA, Bacterial/chemistry; Escherichia coli/genetics; Gene Library; Immunoenzyme Techniques; Molecular Sequence Data; Open Reading Frames; Polymerase Chain Reaction; Protein Sorting Signals/chemistry; Protein Structure, Secondary; Receptors, Cytoplasmic and Nuclear; Sequence Homology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CAMJJ:PEB1C		GO:0051706: physiological interaction between organisms	ECO:0000314:		P		Figure 8 shows PCR of peb1A fragment of both campylobacter strains. It displays the importance of the conservation of the peb1A gene in C. jejuni.	complete CACAO 9600
CAMJJ:PEB1C		GO:0006810: transport	ECO:0000314:		P		Fig. 7 shows southern hybridization of peb1A conversion; Fig. 8 shows PCR of peb1A fragment	complete CACAO 9604

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