User:HLDose

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	ECOLI:NRFA	2014-02-28 11:53:52 CST	GO:0016966 nitric oxide reductase activity (F)	PMID:18245085	ECO:0000314 direct assay evidence used in manual assertion	Protein film voltammetry (PFV) was used to analyze the catalytic activity of the E. coli nrfA gene in reducing NO. Figure 3 shows data for NO reduction for varying concentration of NO by nfrA. Table 1 compares nrfA activity between NO and other substrates.	challenge
updatedbyinstructor	CAMJE:Q0P8Q2	2014-02-28 11:55:03 CST	GO:0009061 anaerobic respiration (P)	PMID:17241202	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3a Wild Type stain of C. jejuni with nrfA gene shows growth under limited oxygen with nitrite, while mutant strain (without nfrA) shows no growth	challenge
updatedbyinstructor	ALLVD:TSDA	2014-02-28 11:55:56 CST	GO:0009055 electron carrier activity (F)	PMID:16995898	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5- Tetrathionate formation from thiosulfate and sulfate formation from thiosulfate; Figure 1- purified thiosulfate and reduce tetrathionate	challenge
unacceptable	NITEU:HAO	2014-02-28 11:58:24 CST	GO:0016491 oxidoreductase activity (F)	PMID:227845	ECO:0000314 direct assay evidence used in manual assertion	Table 1 indicates that the enzyme is reduced in the presence of sodium nitrite	challenge
unacceptable	NITEU:HAO	2014-02-28 11:59:18 CST	GO:0019331 anaerobic respiration, using ammonium as electron donor (P)	PMID:9453157	ECO:0000314 direct assay evidence used in manual assertion	Table 2 and Figure 4 demonstrated hydrazine conversion by the anammox culture	challenge
unacceptable	NITEU:CYCX	2014-02-28 12:00:12 CST	GO:0016491 oxidoreductase activity (F)	PMID:8195067	ECO:0000314 direct assay evidence used in manual assertion	Hydroxylamine is oxidized to nitrite by hydroxylamine oxidoreductase (HAO) in the periplasm of Nitrosomonas europaea (p.3148)	challenge
acceptable	CAMJE:Q0P8Q2	2014-02-28 12:32:21 CST	GO:0016966 cellular detoxification of nitrogen compound (P)	PMID:17241202	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5. Shows the nrfA mutant when compared with the WT strain detoxifies NO under aerobic and anerobic conditions.	challenge
acceptable	CAMJE:Q0P8Q2	2014-03-18 15:54:27 CDT	GO:0042597 periplasmic space (C)	PMID:17241202	ECO:0000314 direct assay evidence used in manual assertion	Mutagenesis of nrfA in C. jejuni resulted in the complete absence of NrfA protein in periplasmic extracts, as evidenced by immunoblotting with cross-reactive anti-E. coli NrfA antibodies (data not shown)	challenge
updatedbyinstructor	NITEU:HAO	2014-03-20 14:08:55 CDT	GO:0047991 hydroxylamine oxidase activity (F)	PMID:836796	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 shows reduction of nitrite synthetase activity of hydraoxalamine oxidoreductase (HAO) in presence of hydrogen peroxide (H2O2).	challenge

acceptable:3
unacceptable:3
requires_changes:0
flagged:0

Annotations challenged by HLDose

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	HLDose, Team DRPs	CAMJE:Q0P8Q2	GO:0016966 - nitric oxide reductase activity (P)	PMID:17241202	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 4
updatedbyinstructor	HLDose, Team DRPs	NITEU:HAO	GO:0047991 - hydroxylamine oxidase activity (F)	PMID:836796	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2

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