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PMID:23727888
| Citation |
Samwer, M, Dehne, HJ, Spira, F, Kollmar, M, Gerlich, DW, Urlaub, H and Görlich, D (2013) The nuclear F-actin interactome of Xenopus oocytes reveals an actin-bundling kinesin that is essential for meiotic cytokinesis. EMBO J. 32:1886-902 |
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| Abstract |
Nuclei of Xenopus laevis oocytes grow 100 000-fold larger in volume than a typical somatic nucleus and require an unusual intranuclear F-actin scaffold for mechanical stability. We now developed a method for mapping F-actin interactomes and identified a comprehensive set of F-actin binders from the oocyte nuclei. Unexpectedly, the most prominent interactor was a novel kinesin termed NabKin (Nuclear and meiotic actin-bundling Kinesin). NabKin not only binds microtubules but also F-actin structures, such as the intranuclear actin bundles in prophase and the contractile actomyosin ring during cytokinesis. The interaction between NabKin and F-actin is negatively regulated by Importin-β and is responsive to spatial information provided by RanGTP. Disconnecting NabKin from F-actin during meiosis caused cytokinesis failure and egg polyploidy. We also found actin-bundling activity in Nabkin's somatic paralogue KIF14, which was previously shown to be essential for somatic cell division. Our data are consistent with the notion that NabKin/KIF14 directly link microtubules with F-actin and that such link is essential for cytokinesis. |
| Links |
PubMed PMC3981176 Online version:10.1038/emboj.2013.108 |
| Keywords |
Actins/metabolism; Actomyosin/metabolism; Animals; Cell Nucleus/metabolism; Chromatography, Affinity; Cytokinesis/physiology; Female; Fluorescent Antibody Technique; Immunoblotting; Kinesin/metabolism; Meiosis/physiology; Microtubules/metabolism; Oocytes/cytology; Oocytes/metabolism; Phalloidine/metabolism; Ploidies; Proteomics; Recombinant Proteins/metabolism; Tandem Mass Spectrometry; Xenopus laevis/growth & development; Xenopus laevis/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0051017: actin filament bundle assembly |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0051017: actin filament bundle assembly |
ECO:0000314: |
P |
Figure 2D shows F-actin interacting with NabKin, resulting in bundling of the F-actin. Compare this image to the control image and you will see that there is a substantially larger amount of bundling in the presence of NabKin |
complete | ||||
Notes
See also
References
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