User:Dakwalea

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	RICPR:Y027	2014-04-06 12:35:51 CDT	GO:0016278 lysine N-methyltransferase activity (F)	PMID:24497633	ECO:0000314 direct assay evidence used in manual assertion	Figure 2c shows the lysine residues with trimethylation sites of Outer membrane protein B.	challenge
updatedbyinstructor	LOCMI:S4W2D1	2014-04-06 13:56:14 CDT	GO:0016246 RNA interference (P)	PMID:23792802	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4d shows how Ago1 knockdown mutants caused far lower Vg expression, impairing vitellogenesis and egg production.	challenge
acceptable	SERMA:HLYB	2014-04-06 15:11:38 CDT	GO:0008320 protein transmembrane transporter activity (F)	PMID:11100827	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1 shows that organisms lacking ShlB had no hemolytic activity since no ShlA was secreted. Mutants with small changes to ShlB were still able to secrete active ShlA.	challenge
requireschanges	SERMA:A1YZD7	2014-04-06 15:54:55 CDT	GO:0019836 hemolysis by symbiont of host erythrocytes (P)	PMID:20003541	ECO:0000315 mutant phenotype evidence used in manual assertion	ShlAB was responsible for direct contact hemolysis. PhlAB was responsible for extracellular hemolysis without contact. Figure 2 shows how mutants lacking PhlAB lost their hemolytic ability without contact. When these cells were given PhlAB, they regained their hemolytic activity without needing contact.	challenge
acceptable	PENCH:D3GE78	2014-04-06 17:55:48 CDT	GO:0047475 phenylacetate-CoA ligase activity (F)	PMID:21625874	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4b and 4c show the minimal activity due to lack of CoA substrate and without purified PhlC. However, when both substrate are present, there was a substantial increase in activity. Figure 2 shows that without PAA present, there are far fewer transcripts for PhlC since PAA and CoA are both needed in order for the protein to perform its ligase activity.	challenge

acceptable:3
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Dakwalea

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	Dakwalea, MichSt14A 30	LOCMI:S4W2D1	GO:0016246 - RNA interference (P)	PMID:23792802	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2 fixed by Dakwalea

1 annotations fixed by Dakwalea

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