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PMID:23792802
| Citation |
Song, J, Guo, W, Jiang, F, Kang, L and Zhou, S (2013) Argonaute 1 is indispensable for juvenile hormone mediated oogenesis in the migratory locust, Locusta migratoria. Insect Biochem. Mol. Biol. 43:879-87 |
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| Abstract |
Juvenile hormone (JH) is the primary hormone controlling vitellogenesis and oocyte maturation in the migratory locust Locusta migratoria, an evolutionarily primitive insect species with panoistic ovaries. However, molecular mechanisms of locust oogenesis remain unclear and the role of microRNA (miRNA) in JH mediated locust vitellogenesis and oocyte maturation has not been explored. Using miRNA sequencing and quantification with small RNA libraries derived from fat bodies of JH-deprived versus JH analog-exposed female adult locusts, we have identified 83 JH up-regulated and 60 JH down-regulated miRNAs. QRT-PCR validation has confirmed that transcription of selected miRNAs responded to JH administration and correlated with changes in endogenous hemolymph JH titers. Depletion of Argonaute 1 (Ago1), a key regulator of miRNA biogenesis and function by RNAi in female adult locusts dramatically decreased the expression of vitellogenin (Vg) and severely impaired follicular epithelium development, terminal oocyte maturation and ovarian growth. Our data indicate that Ago1 and Ago1-dependent miRNAs play a crucial role in locust vitellogenesis and egg production. |
| Links |
PubMed Online version:10.1016/j.ibmb.2013.06.004 |
| Keywords |
Animals; Female; Gene Expression Regulation, Developmental; Insect Proteins/genetics; Insect Proteins/metabolism; Juvenile Hormones/metabolism; Locusta migratoria/embryology; Locusta migratoria/genetics; Locusta migratoria/metabolism; MicroRNAs/genetics; MicroRNAs/metabolism; Ovum/cytology; Ovum/metabolism; Vitellogenesis; Vitellogenins/genetics; Vitellogenins/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0016246: RNA interference |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0016246: RNA interference |
ECO:0000315: |
P |
Figure 4d shows how Ago1 knockdown mutants caused far lower Vg expression, impairing vitellogenesis and egg production. |
complete | ||||
See also
References
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