Category:MichSt14A 30

Figure 4b and 4c show the minimal activity due to lack of CoA substrate and without purified PhlC. However, when both substrate are present, there was a substantial increase in activity. Figure 2 shows that without PAA present, there are far fewer transcripts for PhlC since PAA and CoA are both needed in order for the protein to perform its ligase activity.

ShlAB was responsible for direct contact hemolysis. PhlAB was responsible for extracellular hemolysis without contact. Figure 2 shows how mutants lacking PhlAB lost their hemolytic ability without contact. When these cells were given PhlAB, they regained their hemolytic activity without needing contact.

Figure 1 shows that organisms lacking ShlB had no hemolytic activity since no ShlA was secreted. Mutants with small changes to ShlB were still able to secrete active ShlA.

Figure 4d shows how Ago1 knockdown mutants caused far lower Vg expression, impairing vitellogenesis and egg production.

Figure 2c shows the lysine residues with trimethylation sites of Outer membrane protein B.