GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
|Status||Page||User||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|PENCH:D3GE78||Dakwalea, MichSt14A 30||2014-04-06 17:55:48 CDT||GO:0047475 phenylacetate-CoA ligase activity (F)||PMID:21625874||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 4b and 4c show the minimal activity due to lack of CoA substrate and without purified PhlC. However, when both substrate are present, there was a substantial increase in activity. Figure 2 shows that without PAA present, there are far fewer transcripts for PhlC since PAA and CoA are both needed in order for the protein to perform its ligase activity.
|SERMA:A1YZD7||Dakwalea, MichSt14A 30||2014-04-06 15:54:55 CDT||GO:0019836 hemolysis by symbiont of host erythrocytes (P)||PMID:20003541||ECO:0000315 mutant phenotype evidence used in manual assertion|
ShlAB was responsible for direct contact hemolysis. PhlAB was responsible for extracellular hemolysis without contact. Figure 2 shows how mutants lacking PhlAB lost their hemolytic ability without contact. When these cells were given PhlAB, they regained their hemolytic activity without needing contact.
|SERMA:HLYB||Dakwalea, MichSt14A 30||2014-04-06 15:11:38 CDT||GO:0008320 protein transmembrane transporter activity (F)||PMID:11100827||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 1 shows that organisms lacking ShlB had no hemolytic activity since no ShlA was secreted. Mutants with small changes to ShlB were still able to secrete active ShlA.
|LOCMI:S4W2D1||Dakwalea, MichSt14A 30||2014-04-06 13:56:14 CDT||GO:0016246 RNA interference (P)||PMID:23792802||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 4d shows how Ago1 knockdown mutants caused far lower Vg expression, impairing vitellogenesis and egg production.
|RICPR:Y027||Dakwalea, MichSt14A 30||2014-04-06 12:35:51 CDT||GO:0016278 lysine N-methyltransferase activity (F)||PMID:24497633||ECO:0000314 direct assay evidence used in manual assertion|
Figure 2c shows the lysine residues with trimethylation sites of Outer membrane protein B.
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Pages in category "MichSt14A 30"
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