User:Paxhiami

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	SALTY:RFAF	2012-04-15 17:24:02 CDT	GO:0009244 lipopolysaccharide core region biosynthetic process (P)	PMID:8157607	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 - LPS profile of strains by SDS-PAGE. Mutant phenotype of ∆rfaF (lane 2) was restored to wildtype by complementing with an rfaF plasmid (lane 1).	challenge
acceptable	ECOLI:RFAC	2012-04-14 12:51:15 CDT	GO:0009244 lipopolysaccharide core region biosynthetic process (P)	PMID:8478319	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4a - LPS profile of strains by SDS-PAGE. Mutant phenotype of rfa-2 mutant strain (lane 3) was restored to wildtype by complementing with an rfa-2 plasmid (lane 1).	challenge
requireschanges	ECOLI:RFAQ	2012-04-14 13:54:33 CDT	GO:0008920 lipopolysaccharide heptosyltransferase activity (F)	PMID:9756860	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 - Gas Chromatograph of core oligosaccharide. Compared to wildtype (3a), waaQ mutant (3c) is missing peak 6 (terminal heptose).	challenge
requireschanges	ECOLI:RFAQ	2012-04-14 13:58:39 CDT	GO:0009244 lipopolysaccharide core region biosynthetic process (P)	PMID:9756860	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 - Gas chromatograph of expressed core oligosaccharide. waaQ (3c) mutant expresses a truncated core, missing the terminal heptose (peak 6) as compared to wildtype (3a).	challenge
acceptable	SALTY:RFAC	2012-04-15 17:16:32 CDT	GO:0009244 lipopolysaccharide core region biosynthetic process (P)	PMID:1527014	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5 - LPS profile of strains by SDS-PAGE. Mutant phenotype of ∆rfaC (lane 4) was restored to wildtype by complementing with an rfaC plasmid (lane 5).	challenge

acceptable:3
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Paxhiami

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Ha402057, Team Ferdinand Cohn	ECOLI:ISPG	GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity (ferredoxin) (F)	PMID:16480720	ECO:0000021 physical interaction evidence	challenge	C: 2
requireschanges	Mi896236, Team Stanley Cohen	STRMU:SCRB	GO:0016787 - hydrolase activity (F)	PMID:3009399	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
requireschanges	El001607, Team Kary Mullis	PEDPE:MURI	GO:0016853 - isomerase activity (F)	PMID:1358877	ECO:0000269 experimental evidence used in manual assertion	challenge	C: 2
requireschanges	Napiera8, Team Lappish Hag's Love Potion	DESVH:DCRA	GO:0009454 - aerotaxis (P)	PMID:9202456	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Paxhiami

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	ECOLI:RFAC	2012-03-29 13:40:17 CDT	GO:0008920 lipopolysaccharide heptosyltransferase activity (F)	PMID:11054112	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 - Product in the presence of RfaC contains an additional heptose attached to Kdo2. Product in the absence of RfaC only contains Kdo2.	challenge
acceptable	ECOLI:RFAF	2012-03-29 13:44:35 CDT	GO:0008713 ADP-heptose-lipopolysaccharide heptosyltransferase activity (F)	PMID:11054112	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 - Product in the presence of RfaF contains an additional heptose attached to Hep-Kdo2. Product in the absence of RfaF only contains Hep-Kdo2.	challenge

acceptable:2
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Paxhiami

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Paxhiami