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ECOLI:RFAC
Contents
| Species (Taxon ID) | Escherichia coli (strain K12). (83333) | |
| Gene Name(s) | rfaC (synonyms: rfa-2, waaC, yibC) | |
| Protein Name(s) | Lipopolysaccharide heptosyltransferase 1 | |
| External Links | ||
| UniProt | P24173 | |
| EMBL | M95398 X62530 U00039 U00096 AP009048 | |
| PIR | A40619 | |
| RefSeq | NP_418078.1 YP_491812.1 | |
| PDB | 2H1H | |
| PDBsum | 2H1H | |
| ProteinModelPortal | P24173 | |
| SMR | P24173 | |
| IntAct | P24173 | |
| STRING | 511145.b3621 | |
| CAZy | GT9 | |
| EnsemblBacteria | AAC76645 BAE77671 | |
| GeneID | 12934310 948136 | |
| KEGG | ecj:Y75_p3553 eco:b3621 | |
| PATRIC | 32122729 | |
| EchoBASE | EB1175 | |
| EcoGene | EG11189 | |
| eggNOG | COG0859 | |
| HOGENOM | HOG000257134 | |
| InParanoid | P24173 | |
| KO | K02841 | |
| OMA | WPESHWR | |
| OrthoDB | EOG6WQD45 | |
| PhylomeDB | P24173 | |
| BioCyc | EcoCyc:EG11189-MONOMER ECOL316407:JW3596-MONOMER MetaCyc:EG11189-MONOMER | |
| UniPathway | UPA00958 | |
| EvolutionaryTrace | P24173 | |
| PRO | PR:P24173 | |
| Proteomes | UP000000318 UP000000625 | |
| Genevestigator | P24173 | |
| GO | GO:0008920 GO:0009244 | |
| InterPro | IPR002201 IPR011908 | |
| Pfam | PF01075 | |
| TIGRFAMs | TIGR02193 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0008920 |
lipopolysaccharide heptosyltransferase activity |
ECO:0000314 |
F |
Figure 3 - Product in the presence of RfaC contains an additional heptose attached to Kdo2. Product in the absence of RfaC only contains Kdo2. |
complete | |||||
| GO:0009244 |
lipopolysaccharide core region biosynthetic process |
ECO:0000315 |
P |
Figure 4a - LPS profile of strains by SDS-PAGE. Mutant phenotype of rfa-2 mutant strain (lane 3) was restored to wildtype by complementing with an rfa-2 plasmid (lane 1). |
complete | |||||
|
involved_in |
GO:0009244 |
lipopolysaccharide core region biosynthetic process |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008920 |
lipopolysaccharide heptosyltransferase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009244 |
lipopolysaccharide core region biosynthetic process |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG11189 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0008713 |
ADP-heptose-lipopolysaccharide heptosyltransferase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG12210 |
F |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005829 |
cytosol |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG12210 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0009244 |
lipopolysaccharide core region biosynthetic process |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009244 |
lipopolysaccharide core region biosynthetic process |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008920 |
lipopolysaccharide heptosyltransferase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008920 |
lipopolysaccharide heptosyltransferase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008920 |
lipopolysaccharide heptosyltransferase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009244 |
lipopolysaccharide core region biosynthetic process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016757 |
transferase activity, transferring glycosyl groups |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016740 |
transferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009103 |
lipopolysaccharide biosynthetic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016757 |
transferase activity, transferring glycosyl groups |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009244 |
lipopolysaccharide core region biosynthetic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniPathway:UPA00958 |
P |
Seeded From UniProt |
complete | ||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Gronow, S et al. (2000) Comparative functional characterization in vitro of heptosyltransferase I (WaaC) and II (WaaF) from Escherichia coli. Eur. J. Biochem. 267 6602-11 PubMed GONUTS page
- ↑ 2.0 2.1 Chen, L & Coleman, WG Jr (1993) Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis. J. Bacteriol. 175 2534-40 PubMed GONUTS page
- ↑ 3.0 3.1 3.2 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
- ↑ Beher, MG & Schnaitman, CA (1981) Regulation of the OmpA outer membrane protein of Escherichia coli. J. Bacteriol. 147 972-85 PubMed GONUTS page
- ↑ Coleman, WG Jr & Deshpande, KS (1985) New cysE-pyrE-linked rfa mutation in Escherichia coli K-12 that results in a heptoseless lipopolysaccharide. J. Bacteriol. 161 1209-14 PubMed GONUTS page
- ↑ Kadrmas, JL & Raetz, CR (1998) Enzymatic synthesis of lipopolysaccharide in Escherichia coli. Purification and properties of heptosyltransferase i. J. Biol. Chem. 273 2799-807 PubMed GONUTS page
- ↑ Czyzyk, DJ et al. (2011) Lipopolysaccharide biosynthesis without the lipids: recognition promiscuity of Escherichia coli heptosyltransferase I. Biochemistry 50 10570-2 PubMed GONUTS page
