User:Napiera8

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	ERWAM:Q9XB65	2012-04-09 18:19:13 CDT	GO:0008237 metallopeptidase activity (F)	PMID:10373365	ECO:0000314 direct assay evidence used in manual assertion	Table 3 demonstrates homology with known metalloproteases from other bacteria. Figure 4 confirms proteolytic activity of prtA in Erwinia by demonstrating inhibition by several common protease inhibitors.	challenge
acceptable	CUPMC:CZCD	2012-04-09 20:26:59 CDT	GO:0010312 detoxification of zinc ion (P)	PMID:15516561	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1 shows complementation assay. E. coli with deleted zitB showed high zinc accumulation whereas E. coli with the deletion that were complemented with czcD from R. metallidurans showed lower levels of zinc accumulation.	challenge
requireschanges	RALME:CNRH	2012-04-09 21:05:37 CDT	GO:0006352 transcription initiation, DNA-dependent (P)	PMID:16158236	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 shows results of run off transcription assay. No transcription without cnrH. In the presence of cnrH transcription from cnrC and cnrY is activated.	challenge
requireschanges	GEOSK:D7AF17	2012-04-10 10:39:24 CDT	GO:0016491 oxidoreductase activity (Figures 2 and 3 demonstrate the decreased iron(II) production iron(III)reduction in macA deletion mutants. )	PMID:18616590	IMP: Inferred from Mutant Phenotype		challenge
requireschanges	GEOSN:Q8GGK7	2012-04-10 11:19:50 CDT	GO:0009055 electron carrier activity (F)	PMID:12356333	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5 show the growth rate of both wild type and ppcA mutants when grown with acetate as an electron donor and both fumarate and iron(III) as electron acceptors. The growth rate of the mutant was decreased when Fe(III) was used as an electron acceptor. Combined with Table 3 which illustrates the reduction rates of several compounds in wild type an mutant suggests that ppcA is a carrier of electrons from acetate to iron(III) reductases.	challenge
acceptable	ALCBS:ALKB1	2012-04-10 12:04:57 CDT	GO:0018685 alkane 1-monooxygenase activity (F)	PMID:14871203	ECO:0000315 mutant phenotype evidence used in manual assertion	figure 3 show growth rates of alkB1 mutants on different n-alkanes. the growth rate was lowered in alkB1 mutants grown on n-hexane, while not affected when grown on other alkanes. This shows that alkB1 is involved in the degradation of n-hexane, while other enzymes are involved in the degradation of longer chain alkanes.	challenge
requireschanges	DESVH:DCRA	2012-04-10 12:28:59 CDT	GO:0009454 aerotaxis (P)	PMID:9202456	ECO:0000315 mutant phenotype evidence used in manual assertion	figure 6: dcrA mutants show a decrease in viable cells when exposed to oxygen figure 7: results of oxygen capillary experiment show that dcrA mutants do not migrate from oxygen source compared to wildtype cells.	challenge
acceptable	CUPMC:SODM	2012-04-10 21:26:20 CDT	GO:0004784 superoxide dismutase activity (F)	PMID:12471500	ECO:0000314 direct assay evidence used in manual assertion	Figure 4: ChrC was purified by affinity chromatography. The results of activity stain indicate ChrC is a SOD.	challenge
requireschanges	CLOCL:Q53EH2	2012-04-11 10:35:34 CDT	GO:0030245 cellulose catabolic process (P)	PMID:16930544	ECO:0000314 direct assay evidence used in manual assertion	Figure 1: BglA activity was detected when cellulose was provided as the sole carbon source. No activity detected when grown on other carbon sources.	challenge
acceptable	PSEAI:G8GSY7	2012-04-13 13:08:53 CDT	GO:0003987 acetate-CoA ligase activity (F)	PMID:11577146	ECO:0000315 mutant phenotype evidence used in manual assertion	table 3 shows the specific activity of the ACS protein in various strains when grown on various carbon souces. Strain MS1 showed not ACS activity when grown on any carbon sources. When complemented with a plasmid carrying the acsA gene, ASC activity was detected in MS1	challenge

acceptable:4
unacceptable:0
requires_changes:6
flagged:0

Annotations challenged by Napiera8

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Napiera8

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	PSEOL:ALKK	2012-03-27 19:47:47 CDT	GO:0031956 medium-chain fatty acid-CoA ligase activity (F)	PMID:1453953	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 8, complementation of E. coli acyl-CoA synthetase mutation by plasmid carrying alkK.	challenge

acceptable:0
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Napiera8

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Napiera8