PMID:16930544

Citation	Kosugi, A, Arai, T and Doi, RH (2006) Degradation of cellulosome-produced cello-oligosaccharides by an extracellular non-cellulosomal beta-glucan glucohydrolase, BglA, from Clostridium cellulovorans. Biochem. Biophys. Res. Commun. 349:20-3
Abstract	Clostridium cellulovorans degrades cellulose efficiently to small oligosaccharides, which are used as an energy source. To characterize enzymes related to degrading small oligosaccharides, a gene was cloned for an extracellular non-cellulosomal beta-glucan glucohydrolase (BglA) classified as a family-1 glycosyl hydrolase in C. cellulovorans. Recombinant BglA (rBglA) had higher activity on long glucooligomers than on cellobiose. When cellulosomes and rBglA were incubated with cellulose, the oligosaccharides produced were degraded more effectively to cellobiose and glucose, than with cellulosomes alone, indicating that BglA facilitated the degradation of accessible cello-oligosaccharides produced from cellulose by C. cellulovorans cellulosomes. Thus, this is an example of an extracellular non-cellulosomal enzyme working in a cooperative manner with cellulosomes to degrade cellulose to sugars.
Links	PubMed Online version:10.1016/j.bbrc.2006.07.038
Keywords	Carbohydrates/chemistry; Carbon/chemistry; Cellulase/chemistry; Cellulosomes/chemistry; Clostridium cellulovorans/enzymology; Glucan 1,4-beta-Glucosidase/chemistry; Oligosaccharides/chemistry; Recombinant Proteins/chemistry; beta-Glucans/chemistry
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Significance

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CLOCL:Q53EH2		GO:0030245: cellulose catabolic process	ECO:0000314:		P		Figure 1: BglA activity was detected when cellulose was provided as the sole carbon source. No activity detected when grown on other carbon sources.	complete CACAO 4380

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