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PMID:11577146
| Citation |
Kretzschmar, U, Schobert, M and Görisch, H (2001) The Pseudomonas aeruginosa acsA gene, encoding an acetyl-CoA synthetase, is essential for growth on ethanol. Microbiology (Reading, Engl.) 147:2671-7 |
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| Abstract |
Pseudomonas aeruginosa ATCC 17933 uses a pyrroloquinoline quinone-dependent ethanol oxidation system. Two mutants of P. aeruginosa, unable to grow on ethanol and showing no acetyl-CoA synthetase (ACS) activity under standard test conditions, were complemented by cosmid pTB3018. Subcloning led to the isolation of a gene which encodes a protein with high similarity to acetyl-CoA synthetases. Interruption of the putative acsA gene by a kanamycin-resistance cassette resulted in a mutant also unable to grow on ethanol and with very low residual acetyl-CoA-forming activity. Complementation by the wild-type allele of the acsA gene restored growth and led to the expression of ACS activity in excess of that of wild-type cells. In wild-type P. aeruginosa, ACS activity was induced upon growth on ethanol, 2,3-butanediol, malonate and acetate. The wild-type and mutants defective in ACS activity showed an active acetate kinase (ACK) under the growth conditions used; however, phosphotransacetylase (PTA) could not be detected. The data indicate that P. aeruginosa requires active acsA gene product for growth on ethanol. |
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| Keywords |
Acetates/metabolism; Aldehyde Oxidoreductases/genetics; Aldehyde Oxidoreductases/metabolism; Carbon/metabolism; Cloning, Molecular; Culture Media; Ethanol/metabolism; Gene Deletion; Genes, Bacterial; Genes, Essential; Genetic Complementation Test; Multienzyme Complexes/genetics; Multienzyme Complexes/metabolism; Mutation; Pseudomonas aeruginosa/enzymology; Pseudomonas aeruginosa/genetics; Pseudomonas aeruginosa/growth & development |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003987: acetate-CoA ligase activity |
ECO:0000315: |
F |
table 3 shows the specific activity of the ACS protein in various strains when grown on various carbon souces. Strain MS1 showed not ACS activity when grown on any carbon sources. When complemented with a plasmid carrying the acsA gene, ASC activity was detected in MS1 |
complete | ||||
|
enables |
GO:0003987: acetate-CoA ligase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
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