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PMID:14871203
| Citation |
Hara, A, Baik, SH, Syutsubo, K, Misawa, N, Smits, TH, van Beilen, JB and Harayama, S (2004) Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2. Environ. Microbiol. 6:191-7 |
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| Abstract |
Alcanivorax is an alkane-degrading marine bacterium which propagates and becomes predominant in crude-oil-containing seawater when nitrogen and phosphorus nutrients are supplemented. To identify the genes responsible for alkane degradation in this organism, two putative genes for alkane hydroxylases were cloned from Alcanivorax borkumensis SK2. They were named alkB1 and alkB2. These genes were subsequently disrupted in A. borkumensis SK2, and the growth phenotypes of the disruptants were examined. The results indicate that the alkB1 gene is responsible for the degradation of short-chain n-alkanes. A double mutant defective in both alkB1 and alkB2 was still able to grow on medium-chain n-alkanes, indicating that genes other than alkB1 and alkB2 are also involved in n-alkane hydroxylation by A. borkumensis SK2. |
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| Keywords |
Alkane 1-Monooxygenase/genetics; Alkane 1-Monooxygenase/metabolism; Alkanes/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biodegradation, Environmental; Cloning, Molecular; Cluster Analysis; DNA, Bacterial/chemistry; DNA, Bacterial/isolation & purification; Genes, Bacterial/genetics; Genes, Bacterial/physiology; Halomonadaceae/enzymology; Halomonadaceae/genetics; Halomonadaceae/growth & development; Halomonadaceae/metabolism; Hexanes/metabolism; Molecular Sequence Data; Mutagenesis, Insertional; Octanes/metabolism; Phylogeny; Sequence Analysis, DNA |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0018685: alkane 1-monooxygenase activity |
ECO:0000315: |
F |
figure 3 show growth rates of alkB1 mutants on different n-alkanes. the growth rate was lowered in alkB1 mutants grown on n-hexane, while not affected when grown on other alkanes. This shows that alkB1 is involved in the degradation of n-hexane, while other enzymes are involved in the degradation of longer chain alkanes. |
complete | ||||
|
enables |
GO:0018685: alkane 1-monooxygenase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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