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ECOLI:RFAF

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Species (Taxon ID) Escherichia coli (strain K12). (83333)
Gene Name(s) rfaF (synonyms: waaF)
Protein Name(s) ADP-heptose--LPS heptosyltransferase 2

ADP-heptose--LPS heptosyltransferase II

External Links
UniProt P37692
EMBL U00039
U00096
AP009048
M33577
X62530
PIR S47841
RefSeq NP_418077.1
YP_491813.1
PDB 1PSW
PDBsum 1PSW
ProteinModelPortal P37692
SMR P37692
DIP DIP-10667N
IntAct P37692
STRING 511145.b3620
CAZy GT9
PRIDE P37692
DNASU 948135
EnsemblBacteria AAC76644
BAE77672
GeneID 12934312
948135
KEGG ecj:Y75_p3554
eco:b3620
PATRIC 32122727
EchoBASE EB2124
EcoGene EG12210
eggNOG COG0859
HOGENOM HOG000237541
InParanoid P37692
KO K02843
OMA CLKQRHP
OrthoDB EOG64N9TX
PhylomeDB P37692
BioCyc EcoCyc:EG12210-MONOMER
ECOL316407:JW3595-MONOMER
MetaCyc:EG12210-MONOMER
UniPathway UPA00958
EvolutionaryTrace P37692
PRO PR:P37692
Proteomes UP000000318
UP000000625
Genevestigator P37692
GO GO:0008713
GO:0016757
GO:0009244
InterPro IPR002201
IPR011910
Pfam PF01075
TIGRFAMs TIGR02195

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0008713

ADP-heptose-lipopolysaccharide heptosyltransferase activity

PMID:11054112[1]

ECO:0000314

F

Figure 3 - Product in the presence of RfaF contains an additional heptose attached to Hep-Kdo2. Product in the absence of RfaF only contains Hep-Kdo2.

complete
CACAO 4086

enables

GO:0008713

ADP-heptose-lipopolysaccharide heptosyltransferase activity

PMID:11054112[1]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0009244

lipopolysaccharide core region biosynthetic process

PMID:21873635[2]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG11189
EcoGene:EG11341
EcoGene:EG11423
EcoGene:EG12210
PANTHER:PTN001575314
UniProtKB:G3XD35
UniProtKB:P26469
UniProtKB:P37421
UniProtKB:Q9HUF5

P

Seeded From UniProt

complete

enables

GO:0008713

ADP-heptose-lipopolysaccharide heptosyltransferase activity

PMID:21873635[2]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG12210
PANTHER:PTN001575314

F

Seeded From UniProt

complete

part_of

GO:0005829

cytosol

PMID:21873635[2]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG12210
PANTHER:PTN001575314

C

Seeded From UniProt

complete

involved_in

GO:0009244

lipopolysaccharide core region biosynthetic process

PMID:9266718[3]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0009244

lipopolysaccharide core region biosynthetic process

PMID:15576807[4]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0008713

ADP-heptose-lipopolysaccharide heptosyltransferase activity

PMID:11717579[5]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

part_of

GO:0005829

cytosol

PMID:18304323[6]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005829

cytosol

PMID:17309111[7]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

involved_in

GO:0009103

lipopolysaccharide biosynthetic process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR011910

P

Seeded From UniProt

complete

enables

GO:0016757

transferase activity, transferring glycosyl groups

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR002201
InterPro:IPR011910

F

Seeded From UniProt

complete

involved_in

GO:0009103

lipopolysaccharide biosynthetic process

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0448

P

Seeded From UniProt

complete

enables

GO:0016740

transferase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0808

F

Seeded From UniProt

complete

enables

GO:0016757

transferase activity, transferring glycosyl groups

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0328

F

Seeded From UniProt

complete

involved_in

GO:0009244

lipopolysaccharide core region biosynthetic process

GO_REF:0000041

ECO:0000322

imported manually asserted information used in automatic assertion

UniPathway:UPA00958

P

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 Gronow, S et al. (2000) Comparative functional characterization in vitro of heptosyltransferase I (WaaC) and II (WaaF) from Escherichia coli. Eur. J. Biochem. 267 6602-11 PubMed GONUTS page
  2. 2.0 2.1 2.2 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
  3. Brabetz, W et al. (1997) Deletion of the heptosyltransferase genes rfaC and rfaF in Escherichia coli K-12 results in an Re-type lipopolysaccharide with a high degree of 2-aminoethanol phosphate substitution. Eur. J. Biochem. 247 716-24 PubMed GONUTS page
  4. Joloba, ML et al. (2004) Activation of the gab operon in an RpoS-dependent manner by mutations that truncate the inner core of lipopolysaccharide in Escherichia coli. J. Bacteriol. 186 8542-6 PubMed GONUTS page
  5. Gronow, S et al. (2001) Characterization of the physiological substrate for lipopolysaccharide heptosyltransferases I and II. J. Endotoxin Res. 7 263-70 PubMed GONUTS page
  6. Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed GONUTS page
  7. Zhang, N et al. (2007) Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS. Proteomics 7 484-93 PubMed GONUTS page