User:Ha402057

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	ECOLX:Q2EUT0	2012-04-15 01:08:41 CDT	GO:0003989 acetyl-CoA carboxylase activity (F)	PMID:19965770	ECO:0000315 mutant phenotype evidence used in manual assertion	The effect of the cysteine mutations on enzymatic activity was assessed using a coupled enzyme assay. The kinetic results indicate that mutation of individual cysteine residues 27, 46 and 49 decreases the Vmax only 8- to 11-fold, whereas the Vmax for the C30A variant decreases by over 100-fold. Table 2	challenge
requireschanges	STRPN:ACPS	2012-04-15 01:12:28 CDT	GO:0008897 holo-(acyl-carrier-protein) synthase activity (F)	PMID:16788183	ECO:0000021 physical interaction evidence	fig. 3 details effects of apo-ACP concentrations on activities of S.pneumoniae, E. Coli, & M. Pneumoniae ACP enzymes	challenge
acceptable	ECOLI:FTSP	2012-04-15 01:16:14 CDT	GO:0051301 cell division (P)	PMID:17766410	ECO:0000315 mutant phenotype evidence used in manual assertion	fig. 3 shows addition of multicopy FtsP suppresses division defects of mutants highlighting its significance in cell division	challenge
requireschanges	AQUPY:MURI	2012-04-15 02:05:13 CDT	GO:0008881 glutamate racemase activity (F)	PMID:10331867	ECO:0000315 mutant phenotype evidence used in manual assertion	Structural and mutational analysis allows us to propose a mechanism of metal cofactor-independent glutamate racemase in which 2 cysteine residues are involved in catalysis.	challenge
requireschanges	ECOLI:ISPG	2012-04-15 16:30:22 CDT	GO:0046429 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity (F)	PMID:16480720	ECO:0000021 physical interaction evidence	Table 1 shows conversion of 2-C-methyl-d-eythritol 2,4-cyclodiphosphate into (E)-4-hydroxy-3-methylbut-2-enyl diphosphate by GcpE in the presence of thylakoids and light	challenge

acceptable:1
unacceptable:0
requires_changes:4
flagged:0

Annotations challenged by Ha402057

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Ha402057

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links

acceptable:0
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Ha402057

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Ha402057