GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
Category:Team Stanley Cohen
|Status||Page||User||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|PSEPU:BNZA||Er738332, Team Stanley Cohen||2012-04-15 17:06:14 CDT||GO:0016491 oxidoreductase activity (F)||PMID:8655491||ECO:0000314 direct assay evidence used in manual assertion|
Figure 5 shows the results that toluene is oxidized to cis-toluene dihydrodiol. This was done through thin-layer chromatography, followed by autoradiography to confirm the results seen in table 2.
|HALSA:IDI2||Er738332, Team Stanley Cohen||2012-04-15 17:02:52 CDT||GO:0004452 isopentenyl-diphosphate delta-isomerase activity (F)||PMID:17928693||ECO:0000314 direct assay evidence used in manual assertion|
Figure 1a shows that with the absence of NaCl DMAPP does not form. Figure 2a shows that in the presence of NaCl DMAPP does in fact occur in this species.
|CHLTB:AAXB||Er738332, Team Stanley Cohen||2012-04-15 17:01:59 CDT||GO:0008792 arginine decarboxylase activity (F)||PMID:17693492||ECO:0000314 direct assay evidence used in manual assertion|
Reversed-phase high-pressure liquid chromatography and quantified by their fluorescence intensities are the tests used to see that only l-arginine and its analog l-canavanine were substrates for the enzyme, producing agmatine and γ-guanidinoxypropylamine.
|LACCA:GAL1||Mi896236, Team Stanley Cohen||2012-04-15 16:41:19 CDT||GO:0004335 galactokinase activity (F)||PMID:9603808||ECO:0000315 mutant phenotype evidence used in manual assertion|
Enzymatic assays indicate galK gene is translated into active proteins. Fig 3. shows T7 overexpression of the gal gene products. Fig. 5 shows induction of galK with activity reaching a max after 3 hours.
|STRMU:PT1||Mi896236, Team Stanley Cohen||2012-04-15 15:39:37 CDT||GO:0009401 phosphoenolpyruvate-dependent sugar phosphotransferase system (P)||PMID:8132321||ECO:0000247 sequence alignment evidence used in manual assertion|
Fig. 3 nucleotide sequence of ptsI gene of S. mutans shows 47.7% identity with E1 of E. coli. E1 and HPr products of ptsI genes in S. mutans are also shown to be expressed in E. coli and E1 can complement a ptsI defect in E. coli.
|ECOLI:GYRA||Er738332, Team Stanley Cohen||2012-04-13 13:50:04 CDT||GO:0003918 DNA topoisomerase (ATP-hydrolyzing) activity (F)||PMID:186775||ECO:0000314 direct assay evidence used in manual assertion|
figure 3d shows that a high amount of gryase contained only negative supercoiled DNA.
|STAA8:LACC||Mi896236, Team Stanley Cohen||2012-04-12 13:31:38 CDT||GO:0009024 tagatose-6-phosphate kinase activity (F)||PMID:1655695||ECO:0000247 sequence alignment evidence used in manual assertion|
Fig. 5 shows direct homology of the LacC protein from S. aureus with that of PfkB from E. coli which has evidence of tagatose-6-phosphate kinase activity.
|BACLD:Q65HV3||Er738332, Team Stanley Cohen||2012-04-11 14:17:28 CDT||GO:0008836 diaminopimelate decarboxylase activity (F)||PMID:4972917||ECO:0000314 direct assay evidence used in manual assertion|
figure 2 shows that Meso-DAP conversion to L-lysine was demonstrated by paperchromatography
|STRMU:SCRB||Mi896236, Team Stanley Cohen||2012-03-29 12:21:52 CDT||GO:0016787 hydrolase activity (F)||PMID:3009399||ECO:0000314 direct assay evidence used in manual assertion|
Figures 6 (lanes C,D,E), 7a, and 9.