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Category:Team Stanley Cohen

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesPSEPU:BNZAEr738332, Team Stanley Cohen2012-04-15 17:06:14 CDTGO:0016491 oxidoreductase activity (F)PMID:8655491ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows the results that toluene is oxidized to cis-toluene dihydrodiol. This was done through thin-layer chromatography, followed by autoradiography to confirm the results seen in table 2.

challenge
requireschangesHALSA:IDI2Er738332, Team Stanley Cohen2012-04-15 17:02:52 CDTGO:0004452 isopentenyl-diphosphate delta-isomerase activity (F)PMID:17928693ECO:0000314 direct assay evidence used in manual assertion

Figure 1a shows that with the absence of NaCl DMAPP does not form. Figure 2a shows that in the presence of NaCl DMAPP does in fact occur in this species.

challenge
requireschangesCHLTB:AAXBEr738332, Team Stanley Cohen2012-04-15 17:01:59 CDTGO:0008792 arginine decarboxylase activity (F)PMID:17693492ECO:0000314 direct assay evidence used in manual assertion

Reversed-phase high-pressure liquid chromatography and quantified by their fluorescence intensities are the tests used to see that only l-arginine and its analog l-canavanine were substrates for the enzyme, producing agmatine and γ-guanidinoxypropylamine.

challenge
requireschangesLACCA:GAL1Mi896236, Team Stanley Cohen2012-04-15 16:41:19 CDTGO:0004335 galactokinase activity (F)PMID:9603808ECO:0000315 mutant phenotype evidence used in manual assertion

Enzymatic assays indicate galK gene is translated into active proteins. Fig 3. shows T7 overexpression of the gal gene products. Fig. 5 shows induction of galK with activity reaching a max after 3 hours.

challenge
unacceptableSTRMU:PT1Mi896236, Team Stanley Cohen2012-04-15 15:39:37 CDTGO:0009401 phosphoenolpyruvate-dependent sugar phosphotransferase system (P)PMID:8132321ECO:0000247 sequence alignment evidence used in manual assertion

Fig. 3 nucleotide sequence of ptsI gene of S. mutans shows 47.7% identity with E1 of E. coli. E1 and HPr products of ptsI genes in S. mutans are also shown to be expressed in E. coli and E1 can complement a ptsI defect in E. coli.

challenge
acceptableECOLI:GYRAEr738332, Team Stanley Cohen2012-04-13 13:50:04 CDTGO:0003918 DNA topoisomerase (ATP-hydrolyzing) activity (F)PMID:186775ECO:0000314 direct assay evidence used in manual assertion

figure 3d shows that a high amount of gryase contained only negative supercoiled DNA.

challenge
unacceptableSTAA8:LACCMi896236, Team Stanley Cohen2012-04-12 13:31:38 CDTGO:0009024 tagatose-6-phosphate kinase activity (F)PMID:1655695ECO:0000247 sequence alignment evidence used in manual assertion

Fig. 5 shows direct homology of the LacC protein from S. aureus with that of PfkB from E. coli which has evidence of tagatose-6-phosphate kinase activity.

challenge
acceptableBACLD:Q65HV3Er738332, Team Stanley Cohen2012-04-11 14:17:28 CDTGO:0008836 diaminopimelate decarboxylase activity (F)PMID:4972917ECO:0000314 direct assay evidence used in manual assertion

figure 2 shows that Meso-DAP conversion to L-lysine was demonstrated by paperchromatography

challenge
requireschangesSTRMU:SCRBMi896236, Team Stanley Cohen2012-03-29 12:21:52 CDTGO:0016787 hydrolase activity (F)PMID:3009399ECO:0000314 direct assay evidence used in manual assertion

Figures 6 (lanes C,D,E), 7a, and 9.

challenge

Pages in category "Team Stanley Cohen"

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