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PMID:17928693
| Citation |
Hoshino, T and Eguchi, T (2007) Functional analysis of type 1 isopentenyl diphosphate isomerase from Halobacterium sp. NRC-1. Biosci. Biotechnol. Biochem. 71:2588-91 |
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| Abstract |
Here we report the characterization of the type-1 isopentenyl diphosphate isomerase derived from Halobacterium sp. NRC-1. The expressed purified enzyme showed maximum isomerase activity in the presence of 1 M NaCl at 37 degrees C at pH 6.0. This type-1 enzyme appears to be the first for which the Co2+ ion is required for activity. |
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| Keywords |
Archaeal Proteins/genetics; Archaeal Proteins/isolation & purification; Archaeal Proteins/metabolism; Carbon-Carbon Double Bond Isomerases/genetics; Carbon-Carbon Double Bond Isomerases/isolation & purification; Carbon-Carbon Double Bond Isomerases/metabolism; Cations, Divalent/metabolism; Chromosomes, Bacterial; Cobalt/metabolism; DNA, Bacterial; Escherichia coli/genetics; Flavin Mononucleotide/metabolism; Gene Dosage; Genes, Bacterial; Halobacterium/enzymology; Hydrogen-Ion Concentration; Kinetics; NAD/metabolism; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Sodium Chloride/metabolism; Temperature |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0004452 : isopentenyl-diphosphate delta-isomerase activity |
ECO:0000314: |
F |
Figure 1a shows that with the absence of NaCl DMAPP does not form. Figure 2a shows that in the presence of NaCl DMAPP does in fact occur in this species. |
complete | ||||
See also
References
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