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User:Mi896236

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Spring 2012 Open Competition

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesSTRMU:SCRB2012-03-29 12:21:52 CDTGO:0016787 hydrolase activity (F)PMID:3009399ECO:0000314 direct assay evidence used in manual assertion

Figures 6 (lanes C,D,E), 7a, and 9.

challenge
unacceptableSTAA8:LACC2012-04-12 13:31:38 CDTGO:0009024 tagatose-6-phosphate kinase activity (F)PMID:1655695ECO:0000247 sequence alignment evidence used in manual assertion

Fig. 5 shows direct homology of the LacC protein from S. aureus with that of PfkB from E. coli which has evidence of tagatose-6-phosphate kinase activity.

challenge
unacceptableSTRMU:PT12012-04-15 15:39:37 CDTGO:0009401 phosphoenolpyruvate-dependent sugar phosphotransferase system (P)PMID:8132321ECO:0000247 sequence alignment evidence used in manual assertion

Fig. 3 nucleotide sequence of ptsI gene of S. mutans shows 47.7% identity with E1 of E. coli. E1 and HPr products of ptsI genes in S. mutans are also shown to be expressed in E. coli and E1 can complement a ptsI defect in E. coli.

challenge
requireschangesLACCA:GAL12012-04-15 16:41:19 CDTGO:0004335 galactokinase activity (F)PMID:9603808ECO:0000315 mutant phenotype evidence used in manual assertion

Enzymatic assays indicate galK gene is translated into active proteins. Fig 3. shows T7 overexpression of the gal gene products. Fig. 5 shows induction of galK with activity reaching a max after 3 hours.

challenge

acceptable:0
unacceptable:2
requires_changes:2
flagged:0

Annotations challenged by Mi896236

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Mi896236


Internal Competition only involving UCF students

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableSTRMU:LACC2012-02-24 10:42:31 CSTGO:0009024 tagatose-6-phosphate kinase activity (F)PMID:1400164ECO:0000247 sequence alignment evidence used in manual assertion

Fig. 5 shows significant homology of the LacC protein of S. mutans with that of PfkB from E. coli that has evidence of tagatose-6-phosphate kinase activity.

challenge

acceptable:1
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Mi896236

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Mi896236