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|Status||Page||User||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|NEIME:Q8RMG4||Je507759, Team Golden||2012-03-18 15:24:05 CDT||GO:0006281 DNA repair (P)||PMID:12117927||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 5. The strain MC58 with a wild-type recB allele showed to be less sensitive to the UV254 radiation than the strains MC58-1 and MC58-2 with a defective recB allele. This is represented by the different survival rates shown on Fig. 5b.
|LACCA:Q48518||Al171984, Team Golden||2012-03-18 12:03:12 CDT||GO:0006355 regulation of transcription, DNA-dependent (P)||PMID:15746338||ECO:0000270 expression pattern evidence used in manual assertion|
Figure 2 shows the absorbance and luminescence of cultures of L. Casei with a luciferase gene transcriptionally fused to different genes under the control of separately regulated promoters. The level of expression of the ccpA gene leveled off to zero as the culture grew. Therefore, as the culture grows, the ccpA gene is less expressed due to a decrease in the need for its gene product.
|ECOLI:RECA||Ta992515, Team Golden||2012-03-18 00:54:32 CDT||GO:0006310 DNA recombination (P)||PMID:21912525||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 5B showed all three mutant strains had reduced recombination efficiency of RecA
|LACCA:LDH||Al171984, Team Golden||2012-03-12 10:27:58 CDT||GO:0016491 oxidoreductase activity (F)||PMID:18231816||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 3 shows that the deletion of the ldh1 gene does not contribute to HicD activity
|CLOBH:DNAK||Je507759, Team Golden||2012-02-26 13:46:42 CST||GO:0006950 response to stress (P)||PMID:21378058||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 6 and Table 3. Both show that the dnaK mutants had a maximum growth rate lower than the wild type strain at various stresses (temperature, pH, etc.)
|?||Er292330, Team Golden||2012-02-26 11:54:09 CST||GO:0004386 (helicase assay performed indicates an increase in the amount of RNA released from RNA-DNA duplex with increasing concentrations of rho binding protein (figure 3).)||helicase activity||IDA: Inferred from Direct Assay||challenge|
|STREE:Q9KGU0||Ha856981, Team Golden||2012-02-25 13:27:55 CST||GO:0007155 cell adhesion (P)||PMID:21602213||ECO:0000314 direct assay evidence used in manual assertion|
Figure 6 - Assay demonstrated that "BgaA contributes to the adherence of encapsulated pneumococci to human epithelial cells"
|NEIM8:C9X1X6||Ha856981, Team Golden||2012-02-25 13:06:36 CST||GO:0016337 cell-cell adhesion (cells were monitored prior to infection. No adhesion to the ME180 epithelial cells was observed until the expression of PilC was induced - Figure 3. Both PilC1 and PilC2 induced adhesion to epithelial cells )||PMID:19718432||IDA: Inferred from Direct Assay||challenge|
|STRMU:LACC||Mi896236, Team Golden||2012-02-24 10:42:31 CST||GO:0009024 tagatose-6-phosphate kinase activity (F)||PMID:1400164||ECO:0000247 sequence alignment evidence used in manual assertion|
Fig. 5 shows significant homology of the LacC protein of S. mutans with that of PfkB from E. coli that has evidence of tagatose-6-phosphate kinase activity.
|CITFR:Q5ETW8||Jo436078, Team Golden||2012-02-23 19:58:46 CST||GO:0005351 sugar:proton symporter activity (F)||PMID:9232651||ECO:0000247 sequence alignment evidence used in manual assertion|
Sugar:hydrogen symporter activity based on sequence alignment. See Table 1.
|CITFR:Q5ETW8||Jo436078, Team Golden||2012-02-23 19:58:46 CST||GO:0016021 integral to membrane (C)||PMID:9232651||ECO:0000247 sequence alignment evidence used in manual assertion|
Integral to membrane based on sequence alignment. See Table 1.
|RICPR:TLCE||An553775, Team Golden||2012-02-22 16:35:43 CST||GO:0006810 transport (P)||PMID:16923893||ECO:0000314 direct assay evidence used in manual assertion|
|RICPR:TLCD||An553775, Team Golden||2012-02-22 16:30:29 CST||GO:0006810 transport (P)||PMID:16923893||ECO:0000314 direct assay evidence used in manual assertion|
|RICPR:TLCA||An553775, Team Golden||2012-02-22 16:27:04 CST||GO:0005886 plasma membrane (C)||PMID:1904433||ECO:0000314 direct assay evidence used in manual assertion|
|RICPR:TLCA||An553775, Team Golden||2012-02-22 16:27:04 CST||GO:0006810 transport (P)||PMID:16923893||ECO:0000314 direct assay evidence used in manual assertion|
|RICCO:RICI||Pa491527, Team Golden||2012-02-20 15:39:38 CST||GO:0031640 killing of cells of other organism (P)||PMID:22069759||ECO:0000314 direct assay evidence used in manual assertion|
Ricin toxin binds to the ribosome thereby blocks protein synthesis and cause cell death (Apoptosis). Figure 1 shows a drastic decrease in cell viability with increasing concentration of Ricin.
|HUMAN:TMX1||Pa491527, Team Golden||2012-02-20 15:31:33 CST||GO:0015036 disulfide oxidoreductase activity (F)||PMID:22228764||ECO:0000314 direct assay evidence used in manual assertion|
In order for Ricin to intoxicate the host cell, its disulfide bridge has to be reduced. It has been shown that protein disulfide isomerase (PDI) has the ability to reduce the disulfide bond. However, reduction can still occur even when PDI is depleted, suggesting that other proteins are also responsible to reduce Ricin. Transmenbrane Thioredoxin related protein (TMX) has been found to reduce Ricin. Figure 2 shows that TMX is needed to reduce Ricin. In Lane 1 when TMX is added, Ricin is reduced (the RTA band), whereas in lane 3 with no TMX, Ricin is not reduced.
|LACCA:PTLCB||Te211568, Team Golden||2012-02-09 12:11:29 CST||GO:0016310 phosphorylation (P)||PMID:2125053||ECO:0000315 mutant phenotype evidence used in manual assertion|
Fig. 5 Lanes 1-4 indicate phosphorylation activity.
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