LACCA:PTLCB

Species (Taxon ID)	Lactobacillus casei. (1582)
Gene Name(s)	lacE
Protein Name(s)	PTS system lactose-specific EIICB component EIICB-Lac EII-Lac Lactose permease IIC component PTS system lactose-specific EIIC component Lactose-specific phosphotransferase enzyme IIB component PTS system lactose-specific EIIB component
External Links
UniProt	P24400
EMBL	M60851
PIR	B23697
ProteinModelPortal	P24400
GO	GO:0016021 GO:0005886 GO:0016301 GO:0022869 GO:0046835 GO:0009401 GO:0016310
InterPro	IPR003501 IPR013012 IPR003352 IPR004501 IPR004801
Pfam	PF02378 PF02302
SUPFAM	SSF52794
TIGRFAMs	TIGR00394 TIGR00410 TIGR00853
PROSITE	PS51100 PS51105

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0016310	phosphorylation	PMID:2125053	ECO:0000315			P		Fig. 5 Lanes 1-4 indicate phosphorylation activity.	complete CACAO 3659
	GO:0046835	carbohydrate phosphorylation	PMID:2125053	ECO:0000315			P		As seen in figure 7, lanes 1 through 6, when each cysteine residue was replaced in turn by serine, lactose phosphorylation was found in mutants in which cysteine residues 31, 137, 152, 235, 316, or 349 had been replaced. As seen in figure 7, lane 7, no phosphorylation of the substrate occurred when Cys483 was replaced with a serine. As seen in figure 7, lanes 7 through 9, to confirm that this effect was not due to an undetected secondary mutation, a total of five independently obtained Cys483+Ser483 mutants, from two different experiments, were assayed. It was found in all cases that these mutants did not phosphorylate lactose. As seen in figure 7, lanes 10 through 11, when Cys483 was replaced with histidine, an amino acid found to be involved in all other phosphoryltransfer reactions of the PTS, or tyrosine, no lactose phosphorylation was observed. The loss of phosphorylating activity appeared to be specifically due to the exchange of Cys483 and not due to disruption of the tertiary structure of the protein. Cys483 is the active center phosphorylated amino acid residue in EIIlacL.casei.	complete CACAO 4834
	GO:0005886	plasma membrane	PMID:PMC2753894	ECO:0000315			C		Figure 5: Presence of intact peptidoglycan	complete
involved_in	GO:0046835	carbohydrate phosphorylation	PMID:2125053	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0016310	phosphorylation	PMID:2125053	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0015767	lactose transport	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0022869	P		Seeded From UniProt	complete
involved_in	GO:0034219	carbohydrate transmembrane transport	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0008982	P		Seeded From UniProt	complete
enables	GO:0008982	protein-N(PI)-phosphohistidine-sugar phosphotransferase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR003352 InterPro:IPR003501 InterPro:IPR004501 InterPro:IPR004801 InterPro:IPR013012 InterPro:IPR036095	F		Seeded From UniProt	complete
involved_in	GO:0009401	phosphoenolpyruvate-dependent sugar phosphotransferase system	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR003352 InterPro:IPR003501 InterPro:IPR004501 InterPro:IPR013012 InterPro:IPR036095	P		Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR003352	C		Seeded From UniProt	complete
part_of	GO:0016021	integral component of membrane	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR004501 InterPro:IPR004801	C		Seeded From UniProt	complete
enables	GO:0022869	protein-N(PI)-phosphohistidine-lactose phosphotransferase system transporter activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR004801	F		Seeded From UniProt	complete
part_of	GO:0016021	integral component of membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0812	C		Seeded From UniProt	complete
enables	GO:0016301	kinase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0418	F		Seeded From UniProt	complete
involved_in	GO:0016310	phosphorylation	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0418	P		Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0472	C		Seeded From UniProt	complete
involved_in	GO:0009401	phosphoenolpyruvate-dependent sugar phosphotransferase system	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0598	P		Seeded From UniProt	complete
involved_in	GO:0008643	carbohydrate transport	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0762	P		Seeded From UniProt	complete
enables	GO:0016740	transferase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0808	F		Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1003 UniProtKB-SubCell:SL-0039	C		Seeded From UniProt	complete
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