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User:Pa491527

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Spring 2012 Open Competition

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesECOLX:ELBP2012-03-30 21:09:53 CDTGO:0031640 killing of cells of other organism (P)PMID:12634387ECO:0000314 direct assay evidence used in manual assertion

Figure 3.

challenge
requireschangesCORDI:Q6KE852012-03-30 21:23:29 CDTGO:0031640 killing of cells of other organism (P)PMID:21072261ECO:0000314 direct assay evidence used in manual assertion

Figure 2A.

challenge
requireschangesABRPR:ABRA2012-03-30 21:48:31 CDTGO:0031640 killing of cells of other organism (P)PMID:22228764ECO:0000314 direct assay evidence used in manual assertion

Figure 1B and Table 1.

challenge
acceptableHUMAN:PDIA12012-04-01 12:20:38 CDTGO:0003756 protein disulfide isomerase activity (F)PMID:15225124ECO:0000314 direct assay evidence used in manual assertion

Figure 8 shows the Oxydoreductase activity of PDI using ricin.

challenge
requireschangesECO57:Q7DI682012-04-11 12:31:37 CDTGO:0017148 negative regulation of translation (P)PMID:19139230ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows a decrease level of protein synthesis in presence of shiga toxin 2 (Stx2).

challenge
requireschangesCORDI:Q6KE852012-04-11 12:48:46 CDTGO:0031640 killing of cells of other organism (P)PMID:21567317ECO:0000314 direct assay evidence used in manual assertion

Figure 3.

challenge
requireschangesECO57:Q7DI682012-04-11 12:53:36 CDTGO:0031640 killing of cells of other organism (P)PMID:21567317ECO:0000314 direct assay evidence used in manual assertion

Figure 1.

challenge
requireschangesPSEAE:TOXA2012-04-11 12:56:01 CDTGO:0031640 killing of cells of other organism (P)PMID:21567317ECO:0000314 direct assay evidence used in manual assertion

Figure 3.

challenge
requireschangesECOLX:ELBP2012-04-13 06:46:15 CDTGO:0006915 apoptotic process (P)PMID:12595472ECO:0000314 direct assay evidence used in manual assertion

Figure 8A.

challenge
requireschangesRICCO:RICI2012-04-13 15:55:14 CDTGO:0006915 apoptotic process (P)PMID:11324424ECO:0000314 direct assay evidence used in manual assertion

Figure 4.

challenge
acceptableECOLX:ELBP2012-04-14 09:13:46 CDTGO:0031640 killing of cells of other organism (P)PMID:12595472ECO:0000314 direct assay evidence used in manual assertion

Figure 8A.

challenge
requireschangesRICCO:RICI2012-04-14 09:16:30 CDTGO:0031640 killing of cells of other organism (P)PMID:11324424ECO:0000314 direct assay evidence used in manual assertion

Figure 4.

challenge
requireschangesHUMAN:PDIA12012-04-14 09:18:31 CDTGO:0015036 disulfide oxidoreductase activity (F)PMID:9013867ECO:0000314 direct assay evidence used in manual assertion

Figure 6 (lane 2 and 3). CTA get reduced in the presence of PDI (lane 3). Lane 2 is without PDI.

challenge
requireschangesPSEAI:A4K4472012-04-14 09:25:26 CDTGO:0006915 apoptotic process (P)PMID:22228764ECO:0000314 direct assay evidence used in manual assertion

Figure 1C and table 1.

challenge
requireschangesSAPOF:RIP22012-04-14 09:28:09 CDTGO:0006915 apoptotic process (P)PMID:22228764ECO:0000314 direct assay evidence used in manual assertion

Figure 1D and Table 1.

challenge
acceptableBORPE:CYAA2012-04-14 10:10:54 CDTGO:0007204 elevation of cytosolic calcium ion concentration (P)PMID:17148436ECO:0000314 direct assay evidence used in manual assertion

Figure 1.

challenge
requireschangesBORPE:CYAA2012-04-14 10:20:40 CDTGO:2001170 negative regulation of ATP biosynthetic process (P)PMID:17148436ECO:0000314 direct assay evidence used in manual assertion

Figure 3.

challenge
acceptableBORPE:CYAA2012-04-14 10:24:01 CDTGO:0004016 adenylate cyclase activity (F)PMID:17148436ECO:0000314 direct assay evidence used in manual assertion

Table 1.

challenge
acceptableBORPE:CYAA2012-04-14 10:24:01 CDTGO:0044179 hemolysis in other organism (P)PMID:17148436ECO:0000314 direct assay evidence used in manual assertion

Table 1.

challenge
acceptableSTAAU:TSST2012-04-14 10:41:21 CDTGO:0009405 pathogenesis (P)PMID:22431984ECO:0000314 direct assay evidence used in manual assertion

Figure 1.

challenge

acceptable:6
unacceptable:0
requires_changes:14
flagged:0

Annotations challenged by Pa491527

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesHo701667,
Team Kary Mullis
ECOLI:TYRBGO:0070547 - L-tyrosine aminotransferase activity (F)PMID:3072020ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
requireschangesKimahrom,
Team Free Thinkers
HUMAN:GLSKGO:0004359 - glutaminase activity (F)PMID:10719215ECO:0000247 sequence alignment evidence used in manual assertionchallengeC: 1

0 annotations fixed by Pa491527


Internal Competition only involving UCF students

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableRICCO:RICI2012-02-20 15:39:38 CSTGO:0031640 killing of cells of other organism (P)PMID:22069759ECO:0000314 direct assay evidence used in manual assertion

Ricin toxin binds to the ribosome thereby blocks protein synthesis and cause cell death (Apoptosis). Figure 1 shows a drastic decrease in cell viability with increasing concentration of Ricin.

challenge
acceptableHUMAN:TMX12012-02-20 15:31:33 CSTGO:0015036 disulfide oxidoreductase activity (F)PMID:22228764ECO:0000314 direct assay evidence used in manual assertion

In order for Ricin to intoxicate the host cell, its disulfide bridge has to be reduced. It has been shown that protein disulfide isomerase (PDI) has the ability to reduce the disulfide bond. However, reduction can still occur even when PDI is depleted, suggesting that other proteins are also responsible to reduce Ricin. Transmenbrane Thioredoxin related protein (TMX) has been found to reduce Ricin. Figure 2 shows that TMX is needed to reduce Ricin. In Lane 1 when TMX is added, Ricin is reduced (the RTA band), whereas in lane 3 with no TMX, Ricin is not reduced.

challenge

acceptable:2
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Pa491527

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Pa491527