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User:Ganesani

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SUPER BOWL (Round 5)

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesECOLI:RP542012-04-10 11:14:44 CDTGO:0006355 regulation of transcription, DNA-dependent (P)PMID:21219458ECO:0000270 expression pattern evidence used in manual assertion

Fig. 4: The expression of motility genes fliA, flhC and flhD genes are reduced in rpoN mutants compared to wild type E. coli.

challenge
requireschangesTHEAC:G3LGQ32012-04-10 15:44:45 CDTGO:0050034 L-rhamnose 1-dehydrogenase activity (F)PMID:22481639ECO:0000314 direct assay evidence used in manual assertion

Table 1: enzyme assay shows L-rhamnose activity

challenge
acceptable9SPHN:Q0KJL52012-04-10 15:57:13 CDTGO:0016787 hydrolase activity (F)PMID:22475079ECO:0000314 direct assay evidence used in manual assertion

Fig. 5: pH-rate profile for synthesis of PDC shows hydrolase activity of LigI.

challenge
acceptableENTHI:Q58P262012-04-10 20:14:10 CDTGO:0004586 ornithine decarboxylase activity (F)PMID:22389745ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 10: the four ODC mutants show significantly reduced enzyme activity compared to the wild type ODC.

challenge
acceptableBACPU:F8SVM62012-04-10 20:46:12 CDTGO:0036374 glutathione hydrolase activity (F)PMID:22305170ECO:0000314 direct assay evidence used in manual assertion

Fig. 6: E. coli transformed with GGTBP show gamma-glutamyltransferase activity using many different amino acids acceptors.

challenge
requireschangesPICAD:E7R9692012-04-10 21:15:38 CDTGO:0004022 alcohol dehydrogenase (NAD) activity (Fig. 3C: ADH activity is significantly higher in the wild type strain than in the deletion mutant. )PMID:20635082IMP: Inferred from Mutant Phenotype
challenge
acceptableHUMAN:S39A42012-04-12 21:43:15 CDTGO:0005385 zinc ion transmembrane transporter activity (F)PMID:22242765ECO:0000314 direct assay evidence used in manual assertion

Fig. 2A: Frog oocytes injected with human ZIP4 mRNA showed significant zinc ion uptake activity compared to a negative control.

challenge
requireschangesBORBR:Q7TTE52012-04-13 16:58:23 CDTGO:0016811 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides (F)PMID:22214383ECO:0000314 direct assay evidence used in manual assertion

Fig. 2 shows maleamate hydrolysis activity

challenge

acceptable:4
unacceptable:0
requires_changes:4
flagged:0

Annotations challenged by Ganesani

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesAbdull31,
Team Interior Nebulas
CLOAB:CRTGO:0003859 - 3-hydroxybutyryl-CoA dehydratase activity (F)PMID:18060402ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
requireschangesKr004966,
Team Francis Crick
ECOLI:FTSZGO:0000910 - cytokinesis (P)PMID:8917533ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
requireschangesTh849858,
Team Edward Jenner
ECOLX:Q52R31GO:0005525 - GTP binding (F)PMID:1528267ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
requireschangesMi896236,
Team Stanley Cohen
LACCA:GAL1GO:0004335 - galactokinase activity (F)PMID:9603808ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

0 annotations fixed by Ganesani


MSU Internal

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesECOLI:RPOS2012-03-27 20:06:06 CDTGO:0045809 positive regulation of establishment of competence for transformation (P)PMID:22438941ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4A shows the transformation frequency was greater in the rpoS+ strain than in the rpoS- strain at similar optimal culture densities.

challenge

acceptable:0
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Ganesani

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesAbdull31,
Team Interior Nebulas
CLOAB:CRTGO:0003859 - 3-hydroxybutyryl-CoA dehydratase activity (F)PMID:18060402ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

0 annotations fixed by Ganesani