Category:Team Interior Nebulas

fig. 1: shows extracts from organism in comparison to known products

fig. 2: spectra analysis of enzymatic reactions identifying various products

By comparing table II and III, E. coli transformants show more dehydroquinate synthase activity than wild-type E. coli.

Based on table IV, aroB gene was inserted behind the tac promoter and increase dehydroquinate synthase activity even more.

fig 2: mass spec compares products to identical known molecules

fig 5: degredation of 2,4,6-TCP and increase of 6-CHQ-ox over time

Fig. 2 shows purified uronate dehydrogenase of A. tumefaciens udh gene transformed in E. coli which were analyzed by SDS-PAGE (lane 3)

Table 2 shows turnover numbers and Michaelis constants of uronate dehydrogenase from A. tumefaciens

fig. 1: chloride release with growth turbidity

fig.4: C13-catechol production shown with degradation of CI4- benzene

fig 2.: change in reactants/product levels during hydrolysis

fig 1.: shows purity of beta-xylosidase in comparison to marker proteins

Fig. 4A shows expression of Ugdh in the developing mouse embryo which revealed a single Ugdh transcript of approximately 2.4 kb.

Based on Table II, spectrophotometric measurement indicated that Udpgdh activity of lysates prepared from transfection of a mouse Ugdh expression vector into COS-1 cells was elevated 5–12-fold in comparison to those prepared from vector only, mock-transfected, control cells

Based on fig. 1 (mass spec), two dominant peaks were found after incubation with mannonate dehydratase(ManD). The first peak corresponds to remaining substrate(D-mannonate), whereas the second peak is consistent with formation of the expected protonated dehydration product, 2-KDG (IDA inferred)

Based on table 2, wild type ManD show high affinity to the substrate but the mutant protein Y325F was catalytically inactive while the activity of H311A was only 2.4% that of the native enzyme. The results of site-directed mutagenesis confirmed the functional of these residues in the dehydration reaction (IMP inferred)

Based on fig. 1, hyaluronan(HA) synthase gene was identified based on the fact that Tn insertional mutagenesis caused the band on the gel.

Fig. 4 shows the presence of HasA in E. coli with pPD41-delta5 (lane C) and pPD41-deltaPstI(lane D) while table 1 shows positive HA production for strains that contain the plasmid. Strains with pPD41-deltaPstI show little activity for HA synthesis because no HasB present which is required for the reaction.

table 1 shows UDP-glucose dehydrogenase activity of Streptococcus pyogenes and E. coli with hasB-containing pGAC147 plasmid

Table 3 shows high mannonate oxidoreductase activity by various mutants when functional uxuB gene present in the plasmid.

Table 3 shows high mannonate hydrolyase activity by various mutants when functional uxuA gene present in the plasmid.

Fig. 2 shows overproduction in E. coli, purification and characterization of AbfA.

The recombinant TRII protein expressed in E. coli catalyzes pseudotropine formation from tropinone.

Fig. 4 show relative activity of tropinone.

Fig. 4 shows mass spec. purification of products

Fig. 5 shows concentration vs. time of degradation of PNP, and influx of concentration of associated products

more specifically, the protein involve in Betaine DGTS lipid synthesis

Figure 5 shows DGTS spot on the TLC plates expressed by BTS1

Figure 3B shows 3-hydroxybutyryl-CoA dehydratase (CRT) activity

Table 3 shows positive CRT activity by different strains