User:Abdull31

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	ECOLI:AROB	2012-04-14 13:15:28 CDT	GO:0003856 3-dehydroquinate synthase activity (F)	PMID:6386050	ECO:0000315 mutant phenotype evidence used in manual assertion	By comparing table II and III, E. coli transformants show more dehydroquinate synthase activity than wild-type E. coli. Based on table IV, aroB gene was inserted behind the tac promoter and increase dehydroquinate synthase activity even more.	challenge
acceptable	SOLTU:Q6KF77	2012-04-12 12:54:54 CDT	GO:0050358 tropinone reductase activity (F)	PMID:11855731	ECO:0000314 direct assay evidence used in manual assertion	The recombinant TRII protein expressed in E. coli catalyzes pseudotropine formation from tropinone. Fig. 4 show relative activity of tropinone.	challenge
requireschanges	BACSU:ABFA	2012-04-12 13:28:33 CDT	GO:0046556 alpha-N-arabinofuranosidase activity (F)	PMID:18757805	ECO:0000314 direct assay evidence used in manual assertion	Fig. 2 shows overproduction in E. coli, purification and characterization of AbfA.	challenge
updatedbyinstructor	STRSU:A0A142UME2	2012-04-12 20:15:32 CDT	GO:0008927 mannonate dehydratase activity (F)	PMID:19617363	ECO:0000315 mutant phenotype evidence used in manual assertion	Based on fig. 1 (mass spec), two dominant peaks were found after incubation with mannonate dehydratase(ManD). The first peak corresponds to remaining substrate(D-mannonate), whereas the second peak is consistent with formation of the expected protonated dehydration product, 2-KDG (IDA inferred) Based on table 2, wild type ManD show high affinity to the substrate but the mutant protein Y325F was catalytically inactive while the activity of H311A was only 2.4% that of the native enzyme. The results of site-directed mutagenesis confirmed the functional of these residues in the dehydration reaction (IMP inferred)	challenge
acceptable	ECOLI:UXUA	2012-04-12 18:32:43 CDT	GO:0008927 mannonate dehydratase activity (F)	PMID:6997264	ECO:0000315 mutant phenotype evidence used in manual assertion	Table 3 shows high mannonate hydrolyase activity by various mutants when functional uxuA gene present in the plasmid.	challenge
acceptable	ECOLI:UXUB	2012-04-12 18:34:33 CDT	GO:0008866 fructuronate reductase activity (F)	PMID:6997264	ECO:0000315 mutant phenotype evidence used in manual assertion	Table 3 shows high mannonate oxidoreductase activity by various mutants when functional uxuB gene present in the plasmid.	challenge
acceptable	STRPY:UDG	2012-04-12 19:34:51 CDT	GO:0003979 UDP-glucose 6-dehydrogenase activity (F)	PMID:8463246	ECO:0000314 direct assay evidence used in manual assertion	table 1 shows UDP-glucose dehydrogenase activity of Streptococcus pyogenes and E. coli with hasB-containing pGAC147 plasmid	challenge
unacceptable	STRPY:HASA	2012-04-12 20:13:49 CDT	GO:0050501 hyaluronan synthase activity (F)	PMID:8366070	ECO:0000315 mutant phenotype evidence used in manual assertion	Based on fig. 1, hyaluronan(HA) synthase gene was identified based on the fact that Tn insertional mutagenesis caused the band on the gel. Fig. 4 shows the presence of HasA in E. coli with pPD41-delta5 (lane C) and pPD41-deltaPstI(lane D) while table 1 shows positive HA production for strains that contain the plasmid. Strains with pPD41-deltaPstI show little activity for HA synthesis because no HasB present which is required for the reaction.	challenge
acceptable	MOUSE:UGDH	2012-04-12 21:04:09 CDT	GO:0003979 UDP-glucose 6-dehydrogenase activity (F)	PMID:9737970	ECO:0000314 direct assay evidence used in manual assertion	Fig. 4A shows expression of Ugdh in the developing mouse embryo which revealed a single Ugdh transcript of approximately 2.4 kb. Based on Table II, spectrophotometric measurement indicated that Udpgdh activity of lysates prepared from transfection of a mouse Ugdh expression vector into COS-1 cells was elevated 5–12-fold in comparison to those prepared from vector only, mock-transfected, control cells	challenge
acceptable	AGRFC:URODH	2012-04-13 17:58:39 CDT	GO:0050388 uronate dehydrogenase activity (F)	PMID:19060141	ECO:0000314 direct assay evidence used in manual assertion	Fig. 2 shows purified uronate dehydrogenase of A. tumefaciens udh gene transformed in E. coli which were analyzed by SDS-PAGE (lane 3) Table 2 shows turnover numbers and Michaelis constants of uronate dehydrogenase from A. tumefaciens	challenge

acceptable:7
unacceptable:1
requires_changes:1
flagged:0

Annotations challenged by Abdull31

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Abdull31

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	CHLRE:Q6DN05	2012-04-01 15:42:39 CDT	GO:0006578 betaine biosynthetic process (P)	PMID:15701786	ECO:0000315 mutant phenotype evidence used in manual assertion	more specifically, the protein involve in Betaine DGTS lipid synthesis Figure 5 shows DGTS spot on the TLC plates expressed by BTS1	challenge
requireschanges	CLOAB:CRT	2012-03-30 18:13:08 CDT	GO:0003859 3-hydroxybutyryl-CoA dehydratase activity (F)	PMID:18060402	ECO:0000314 direct assay evidence used in manual assertion	Figure 3B shows 3-hydroxybutyryl-CoA dehydratase (CRT) activity Table 3 shows positive CRT activity by different strains	challenge

acceptable:1
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Abdull31

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Abdull31