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PMID:9737970

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Citation

Spicer, AP, Kaback, LA, Smith, TJ and Seldin, MF (1998) Molecular cloning and characterization of the human and mouse UDP-glucose dehydrogenase genes. J. Biol. Chem. 273:25117-24

Abstract

The enzyme UDP-glucose dehydrogenase (Udpgdh) (EC 1.1.1.22) converts UDP-glucose to UDP-glucuronate, a critical component of the glycosaminoglycans, hyaluronan, chondroitin sulfate, and heparan sulfate. Although Udpgdh is a comparatively well characterized enzyme, no vertebrate genes encoding this enzyme have been reported to date. We report the cloning and characterization of the human and mouse UDP-glucose dehydrogenase genes. Mouse and human cDNAs predicted proteins of 493 and 494 amino acids, 24-25 residues longer at their carboxyl termini than the previously reported bovine Udpgdh sequence. The mouse Ugdh gene is composed of 10 exons, spanning 15 kilobases. Northern analyses indicated widespread expression of the gene in embryo and adult. Through interspecific backcross analyses, we localized the Ugdh gene to mouse chromosome 5 at approximately 39 centimorgans, suggesting that the human UGDH gene is localized to chromosome 4p13-15. Results from Southern analyses strongly suggest that Udpgdh is encoded by a single gene in the mouse. Transfection of mouse Ugdh expression vectors led to an increase in detectable Udpgdh activity in mammalian cells. Preliminary expression studies indicated that proinflammatory cytokines, such as interleukin 1beta, can substantially increase the expression of human UGDH in cultured human fibroblasts, suggesting that glycosaminoglycan biosynthesis may be partly regulated by the availability of activated UDP-glucuronate, as determined by relative Udpgdh expression levels.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; Cells, Cultured; Chromosome Mapping; Cloning, Molecular; DNA, Complementary; Humans; Mice; Molecular Sequence Data; RNA, Messenger/genetics; Sequence Homology, Amino Acid; Uridine Diphosphate Glucose Dehydrogenase/genetics; Uridine Diphosphate Glucose Dehydrogenase/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:UGDH

GO:0003979: UDP-glucose 6-dehydrogenase activity

ECO:0000314:

F

Fig. 4A shows expression of Ugdh in the developing mouse embryo which revealed a single Ugdh transcript of approximately 2.4 kb.

Based on Table II, spectrophotometric measurement indicated that Udpgdh activity of lysates prepared from transfection of a mouse Ugdh expression vector into COS-1 cells was elevated 5–12-fold in comparison to those prepared from vector only, mock-transfected, control cells

complete
CACAO 4498

MOUSE:UGDH

enables

GO:0003979: UDP-glucose 6-dehydrogenase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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