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PMID:8463246
| Citation |
Dougherty, BA and van de Rijn, I (1993) Molecular characterization of hasB from an operon required for hyaluronic acid synthesis in group A streptococci. Demonstration of UDP-glucose dehydrogenase activity. J. Biol. Chem. 268:7118-24 |
|---|---|
| Abstract |
The membrane-associated hyaluronate synthase produces capsular hyaluronate in group A streptococci by the alternate addition of UDP-N-acetylglucosamine and UDP-glucuronic acid. Previous studies identified a locus required for hyaluronate synthase activity and suggested that a gene involved in the production of UDP-glucuronic acid (UDP-glucose dehydrogenase) also mapped to the locus. In the present study the putative UDP-glucose dehydrogenase gene (hasB) was cloned and the DNA sequence determined. The hasB gene product was shown to have global similarity with AlgD, a dehydrogenase, which catalyzes the production of GDP-mannuronic acid for the alginate capsule of Pseudomonas aeruginosa. Regions of local homology have been identified which apparently correspond to the NAD-binding and enzyme active sites of HasB and AlgD. In order to show that hasB expression correlated with UDP-glucose dehydrogenase activity, the hasB gene was cloned under control of the T7 promoter. Hyperexpression of hasB resulted in a protein of approximately 47 kDa and high levels of UDP-glucose dehydrogenase activity were observed. These data demonstrate that hasB encodes the UDP-glucose dehydrogenase of group A streptococci. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Carbohydrate Dehydrogenases/genetics; Cloning, Molecular; DNA, Bacterial; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Hyaluronic Acid/biosynthesis; Molecular Sequence Data; Mutagenesis, Insertional; Operon; Restriction Mapping; Sequence Homology, Amino Acid; Streptococcus/enzymology; Streptococcus/genetics; Uridine Diphosphate Glucose Dehydrogenase/genetics; Uridine Diphosphate Glucose Dehydrogenase/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003979 : UDP-glucose 6-dehydrogenase activity |
ECO:0000314: |
F |
table 1 shows UDP-glucose dehydrogenase activity of Streptococcus pyogenes and E. coli with hasB-containing pGAC147 plasmid |
complete | ||||
|
enables |
GO:0003979: UDP-glucose 6-dehydrogenase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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