GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search

Inui, M, Suda, M, Kimura, S, Yasuda, K, Suzuki, H, Toda, H, Yamamoto, S, Okino, S, Suzuki, N and Yukawa, H (2008) Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli. Appl. Microbiol. Biotechnol. 77:1305-16


A recombinant butanol pathway composed of Clostridium acetobutylicum ATCC 824 genes, thiL, hbd, crt, bcd-etfB-etfA, and adhe1 (or adhe) coding for acetyl-CoA acetyltransferase (THL), beta-hydroxybutyryl-CoA dehydrogenase (HBD), 3-hydroxybutyryl-CoA dehydratase (CRT), butyryl-CoA dehydrogenase (BCD), butyraldehyde dehydrogenase (BYDH), and butanol dehydrogenase (BDH), under the tac promoter control was constructed and was introduced into Escherichia coli. The functional expression of these six enzymes was proved by demonstrating the corresponding enzyme activities using spectrophotometric, high performance liquid chromatography and gas chromatography analyses. The BCD activity, which was not detected in E. coli previously, was shown in the present study by performing the procedure from cell extract preparation to activity measurement under anaerobic condition. Moreover, the etfA and etfB co-expression was found to be essential for the BCD activity. In the case of BYDH activity, the adhe gene product was shown to have higher specificity towards butyryl-CoA compared to the adhe1 product. Butanol production from glucose was achieved by the highly concentrated cells of the butanologenic E. coli strains, BUT1 with adhe1 and BUT2 with adhe, under anaerobic condition, and the BUT1 and BUT2 strains were shown to produce 4 and 16-mM butanol with 6- and 1-mM butyrate as a byproduct, respectively. This study reports the novel butanol production by an aerobically pregrown microorganism possessing the genes of a strict anaerobe, Clostridium acetobutylicum.


PubMed Online version:10.1007/s00253-007-1257-5


3-Hydroxyacyl CoA Dehydrogenases/genetics; 3-Hydroxyacyl CoA Dehydrogenases/metabolism; Acetyl-CoA C-Acetyltransferase/genetics; Acetyl-CoA C-Acetyltransferase/metabolism; Acyl Coenzyme A/genetics; Acyl Coenzyme A/metabolism; Alcohol Oxidoreductases/genetics; Alcohol Oxidoreductases/metabolism; Aldehyde Oxidoreductases/genetics; Aldehyde Oxidoreductases/metabolism; Biosynthetic Pathways; Butanols/metabolism; Butyryl-CoA Dehydrogenase/genetics; Butyryl-CoA Dehydrogenase/metabolism; Clostridium acetobutylicum/enzymology; Clostridium acetobutylicum/genetics; Escherichia coli/genetics; Genes, Bacterial; Genetic Engineering; Recombinant Proteins/genetics; Recombinant Proteins/metabolism



Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status


GO:0003859 : 3-hydroxybutyryl-CoA dehydratase activity



Figure 3B shows 3-hydroxybutyryl-CoA dehydratase (CRT) activity

Table 3 shows positive CRT activity by different strains

CACAO 4109

See also


See Help:References for how to manage references in GONUTS.