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User:Dixonkev

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SUPER BOWL (Round 5)

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptablePSEWB:PNPA2012-04-12 11:21:56 CDTGO:0004497 monooxygenase activity (F)PMID:19218392ECO:0000314 direct assay evidence used in manual assertion

Fig. 4 shows mass spec. purification of products

Fig. 5 shows concentration vs. time of degradation of PNP, and influx of concentration of associated products

challenge
requireschangesHYPJE:Q924582012-04-13 12:12:24 CDTGO:0005975 carbohydrate metabolic process (P)PMID:9020869ECO:0000314 direct assay evidence used in manual assertion

fig 2.: change in reactants/product levels during hydrolysis

fig 1.: shows purity of beta-xylosidase in comparison to marker proteins

challenge
requireschanges9BURK:O692642012-04-13 17:44:04 CDTGO:0000166 nucleotide binding (F)PMID:9249026ECO:0000314 direct assay evidence used in manual assertion

fig. 1: chloride release with growth turbidity

fig.4: C13-catechol production shown with degradation of CI4- benzene

challenge
requireschangesCUPNE:Q8KTD62012-04-13 21:01:36 CDTGO:0009712 catechol-containing compound metabolic process (P)PMID:12057943ECO:0000314 direct assay evidence used in manual assertion

fig 2: mass spec compares products to identical known molecules

fig 5: degredation of 2,4,6-TCP and increase of 6-CHQ-ox over time

challenge
requireschangesPSEPU:Q9Z4172012-04-14 16:09:30 CDTGO:0006725 cellular aromatic compound metabolic process (P)PMID:9440519ECO:0000314 direct assay evidence used in manual assertion

fig. 1: shows extracts from organism in comparison to known products

fig. 2: spectra analysis of enzymatic reactions identifying various products

challenge

acceptable:1
unacceptable:0
requires_changes:4
flagged:0

Annotations challenged by Dixonkev

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesHa856981,
Team Martha Chase
BACAN:Q81JD7GO:0006958 - complement activation, classical pathway (P)PMID:22442442ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

0 annotations fixed by Dixonkev


MSU Internal

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptable?2012-04-01 12:10:58 CDT GO:0018584 (Fig 1: uses lac promoter and subcloning shows ability to form TCP associated with inserted genes Fig 3: autoradiogram of gel maps expression of tftA after insertion in various host cells)2,4,5-trichlorophenoxyacetic acid oxygenase activityIEP: Inferred from Expression Pattern
challenge

acceptable:0
unacceptable:1
requires_changes:0
flagged:0

Annotations challenged by Dixonkev

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Dixonkev