User:Kr004966

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	BACSU:FTSA	2012-03-27 11:59:55 CDT	GO:0016887 ATPase activity (F)	PMID:11298280	ECO:0000314 direct assay evidence used in manual assertion	Shown that B. subtilis FtsA is capable of binding and hydrolysing ATP (Fig. 7), To test whether FtsA has ATPase activity, a measurement of the hydrolysis of ATP by monitoring the release of 32Pi from [γ-32P]-ATP. The activity was readily detected, and analysis of the reaction revealed Michaelis–Menten kinetics (Fig. 7B) with a Km of 0.7 mM and a Vmax of 0.125 mol of ATP hydrolysed per second per mol of FtsA dimer.	challenge
acceptable	BACSU:SEPF	2012-03-29 11:32:42 CDT	GO:0090529 cell septum assembly (P)	PMID:16420366	ECO:0000315 mutant phenotype evidence used in manual assertion	Deletion leads to impaired septa formation Fig 2, as well as slowed growth.	challenge
requireschanges	ECOLX:SULA	2012-03-29 11:51:35 CDT	GO:0032466 negative regulation of cytokinesis (P)	PMID:22432817	ECO:0000315 mutant phenotype evidence used in manual assertion	SulA inhibits the assembly of FtsZ, preventing the cells from performing cytokenesis. Figure 8E showing the increase in SulA,causes a decrease in FtsZ formation. Figure 6 EM images of PaFtsZ and the inhibition of assemble by SulA. Figure 1 (a,b) EM images of ECFtsZ without SulA and with the indicated addition of SulA	challenge
requireschanges	ECOLX:E0WA88	2012-03-30 12:34:21 CDT	GO:0009279 cell outer membrane (C)	PMID:1370823	ECO:0000315 mutant phenotype evidence used in manual assertion	Furthermore, the experiment using the OmpA-deficient mutant HN742 (Fig. 6) showed conclusively that the channel formation was caused by OmpA, denaturation of OmpA essentially abolished the solute diffusion process and that nonporin proteins such as bovine serum albumin could not facilitate the solute diffusion across the membrane.	challenge
requireschanges	ECOLI:FTSZ	2012-04-09 15:57:38 CDT	GO:0000910 cytokinesis (P)	PMID:8917533	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2b and C FtsZ labeled with GDP for flourences, shows formation of ring during intermediate and late stages of division.	challenge
	ECOLI:FTSZ	2015-02-03 18:12:45 CST	GO:0000910 cytokinesis (P)	PMID:8917533	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2b and C FtsZ labeled with GDP for flourences, shows formation of ring during intermediate and late stages of division, indication of involvement in cytokinesis.	challenge

acceptable:2
unacceptable:0
requires_changes:3
flagged:0

Annotations challenged by Kr004966

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Kr004966

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	STRP2:Q04LN6	2012-02-09 12:04:33 CST	GO:0009986 cell surface (C)	PMID:21602213	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig.1 showing relative adherence of mutants with mutations in the bgaA gene protein product, indication that the protein is found on cell surface, due to its involvement in cell to cell binding.	challenge
updatedbyinstructor	STREE:Q9KGU0	2012-02-22 08:57:20 CST	GO:0016787 hydrolase activity (F)	PMID:21602213	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2b indicates evidence of hydrolase activity, more specificly beta- galactosidase activity.	challenge
requireschanges	ECOLI:FTSZ	2012-02-22 11:12:39 CST	GO:0051301 cell division (P)	PMID:8917533	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2b and C FtsZ labeled with GDP for flourences, shows formation of ring during intermediate and late stages of division.	challenge
requireschanges	BACSU:CATE	2012-03-13 12:41:03 CDT	GO:0004096 catalase activity (F)	PMID:16151211	ECO:0000315 mutant phenotype evidence used in manual assertion	A clonned KatE gene, inserted in a fermenting bacteria L. lactis was able to produce a catalase and survive in aerobic conditions. Refer to Figure 1. L. Lactis produces an active catalase KatE, and were mixed with H2O2 solution. Catalase activity was detected by bubble formation due to O2 liberation.	challenge
requireschanges	SALTY:MGLA	2012-03-15 18:08:29 CDT	GO:0016887 ATPase activity (F)	PMID:8387496	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3, showing that MglA had ATPase activity. MglA is specifically stimulated by galactose, while being uneffected by other compounds. Indication that the ATPase supports the implication of hydrolysis in protein-dependent transport system. Also figure 2 shows the peak of ATPase activity eluted from column that coincides with the peak of MglA protein.	challenge

acceptable:0
unacceptable:0
requires_changes:4
flagged:0

Annotations challenged by Kr004966

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Kr004966