User:Fclingen

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	HUMAN:BCAM	2014-01-26 19:14:08 CST	GO:0008022 protein C-terminus binding (F)	PMID:24453976	ECO:0000314 direct assay evidence used in manual assertion	Cells not expressing Lu/BCAM did not respond to CNF1 toxin. The data indicated that the toxin (CNF1) specifically binds to Lu/BCAM (figure 4). MALDI-TOF analysis of the precipitate identified Lu/BCAM as an interaction partner for CNF1. It was suggested that there are two interaction sites in CNF1: The N-terminus binding to 37LRP/67LR and the C-terminus binding with high affinity to Lu/BCAM. The interaction of CNF1 Lu/BCAM was verified using several methods: Western blotting, immunofluorescence, MALDI-TOF analysis, and fluorescence activated cell sorting (FACS) analysis.	challenge
unacceptable	HUMAN:EAF1	2014-01-27 14:42:19 CST	GO:0030097 hemopoiesis (P)	PMID:24445282	ECO:0000314 direct assay evidence used in manual assertion	Figure 1. Eaf1 promotes hematopoiesis.	challenge
unacceptable	HUMAN:EAF1	2014-01-28 18:33:01 CST	GO:0010629 negative regulation of gene expression (P)	PMID:24445282	ECO:0000314 direct assay evidence used in manual assertion	Figure 3. Eaf1 suppresses human FOXO 3a expression.	challenge
unacceptable	ECOLI:HHA	2014-01-28 19:41:01 CST	GO:0061435 positive regulation of transcription from a mobile element promoter (P)	PMID:24465756	ECO:0000314 direct assay evidence used in manual assertion	Figure 1. Hha ehances transcription to promote motility.	challenge
unacceptable	ECOLI:QSEC	2014-01-28 19:47:40 CST	GO:0061435 positive regulation of transcription from a mobile element promoter (P)	PMID:24465756	ECO:0000314 direct assay evidence used in manual assertion	Figure 1. qseC enhances transcription to promote motility.	challenge
updatedbyinstructor	ECOLI:LAMB	2014-01-28 20:19:27 CST	GO:0015288 porin activity (F)	PMID:24412198	ECO:0000314 direct assay evidence used in manual assertion	Fig 6 (top) has data for lamB	challenge
unacceptable	ECOLI:ODP1	2014-01-28 20:24:13 CST	GO:0071236 cellular response to antibiotic (P)	PMID:24412198	ECO:0000314 direct assay evidence used in manual assertion	Figure 3. ODP1 decreases in antibiotic resistant strains.	challenge
unacceptable	HUMAN:BRD4	2014-01-28 21:13:57 CST	GO:0008284 positive regulation of transcription during mitosis (P)	PMID:24403256	ECO:0000314 direct assay evidence used in manual assertion	Figure 1. BRD4 promotes proliferation in acute myeloid leukaemia cells.	challenge

acceptable:0
unacceptable:6
requires_changes:0
flagged:0

Annotations challenged by Fclingen

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Kdiawara, Team Lobster Soul	HUMAN:CH60	GO:0046789 - host cell surface receptor binding (F)	PMID:12496435	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 4
updatedbyinstructor	Grolfe, Team Pancakes At Nite	HUMAN:CIB1	GO:0008427 - calcium-dependent protein kinase inhibitor activity (F)	PMID:20473878	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1 fixed by Fclingen
unacceptable	Kyoung, Team Pnut Butter Squares	MOUSE:O54716	GO:0006903 - vesicle targeting (P)	PMID:9422766	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
unacceptable	Grolfe, Team Pancakes At Nite	HUMAN:CIB1	GO:0008427 - calcium-dependent protein kinase inhibitor activity (F)	PMID:20473878	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3
unacceptable	Sirons, Team Takin This Outside	HUMAN:TAU	GO:0031113 - regulation of microtubule polymerization (P)	PMID:1057175	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1

1 annotations fixed by Fclingen

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Annotations challenged by Fclingen

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Fclingen

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