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PMID:9422766

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Citation

Chen, J, Vijayakumar, S, Li, X and Al-Awqati, Q (1998) Kanadaptin is a protein that interacts with the kidney but not the erythroid form of band 3. J. Biol. Chem. 273:1038-43

Abstract

Although epithelial membrane proteins are separately targeted to apical or basolateral domains, some are apically located in one cell type but are basolateral in others. More dramatically, the anion exchanger of a clonal cell line of intercalated cells derived from the kidney can be retargeted from the apical to basolateral domain. This Cl:HCO3 exchanger, kAE1, is an alternately spliced form of the erythroid anion exchanger (AE1, band 3), but unlike band 3 it does not bind ankyrin. Here we identify a new protein (kanadaptin) that binds to the cytoplasmic domain of kAE1 in vitro and in vivo but not to the erythroid AE1 or to ankyrin. No significant homologous proteins have been reported so far. Kanadaptin is widely expressed in epithelial (kidney, lung, and liver) and non-epithelial cells (brain and skeletal and cardiac muscle). In kidney, we found by immunocytochemistry that kanadaptin was only expressed in the collecting tubule. In the intercalated cells of this segment, it colocalized with kAE1 in cytoplasmic vesicles but not when the exchanger was in the basolateral membrane. These results raised the possibility that this protein is involved in the targeting of kAE1 vesicles to their final destination.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Anion Exchange Protein 1, Erythrocyte/metabolism; Antiporters; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Glutathione Transferase/genetics; Glutathione Transferase/metabolism; Immunohistochemistry; Kidney/metabolism; Molecular Sequence Data; Protein Binding; Rabbits; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Saccharomyces cerevisiae/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:A0A0J9YTU2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P04919-2

F

Seeded From UniProt

complete

MOUSE:A0A0J9YU76

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P04919-2

F

Seeded From UniProt

complete

MOUSE:A0A0J9YUT6

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P04919-2

F

Seeded From UniProt

complete

MOUSE:E9PX68

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P04919-2

F

Seeded From UniProt

complete

MOUSE:E9Q1Q0

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P04919-2

F

Seeded From UniProt

complete

MOUSE:E9Q585

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P04919-2

F

Seeded From UniProt

complete

MOUSE:O54716

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P04919-2

F

Seeded From UniProt

complete

MOUSE:Q3UHY0

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P04919-2

F

Seeded From UniProt

complete

MOUSE:Q3UUI2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P04919-2

F

Seeded From UniProt

complete

MOUSE:Q5BKS1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P04919-2

F

Seeded From UniProt

complete

MOUSE:Q6GQW1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P04919-2

F

Seeded From UniProt

complete

MOUSE:Q7TNP8

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P04919-2

F

Seeded From UniProt

complete

MOUSE:O54716

NOT

GO:0006903: vesicle targeting

ECO:0000314:

P

Figure 5

complete
CACAO 9255


See also

References

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