User:Willcoxe

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	ASFB7:P12	2013-02-04 22:24:35 CST	GO:0019012 virion (C)	PMID:8350406	ECO:0000314 direct assay evidence used in manual assertion	As seen in Figure 1, immunoprecipitation of protein p12 of infected Vero cells and purified virions are compared, demonstrating that the function of p12 is to attach ASFV to a host cell receptor.	challenge
acceptable	ASFB7:P12	2013-02-04 22:24:35 CST	GO:0019062 viral attachment to host cell (P)	PMID:8350406	ECO:0000314 direct assay evidence used in manual assertion	As seen in Figure 6, when purified p12 is bound to the cell receptor, ASFV is unable to bind to the receptor. This is shown when the cell monolayers are washed with PBS and radiolabeled (radioactive I-125) ASFV is washed away, decreasing radioactivity.	challenge
updatedbyinstructor	ASFB7:DPOLX	2013-02-07 23:33:09 CST	GO:0003887 DNA-directed DNA polymerase activity (F)	PMID:9388236	ECO:0000314 direct assay evidence used in manual assertion	Figure 4 shows that ASFV pol X catalyzes template-directed DNA synthesis.	challenge
acceptable	ASFB7:IAP	2013-02-08 10:51:18 CST	GO:0039650 suppression by virus of host cysteine-type endopeptidase activity involved in apoptotic process (P)	PMID:11222676	ECO:0000314 direct assay evidence used in manual assertion	As seen in Figure 5, viral IAP inhibits host cell apopotosis by modulating caspase-3 activity within the cell. This was determined by comparing the levels of caspase-3 activity in Vero cells infected with the deletion mutant ΔIAP and the parental virus BA71V, using a caspase-specific assay	challenge
updatedbyinstructor	FOWPN:RUVV	2013-03-06 14:44:06 CST	GO:0008821 crossover junction endodeoxyribonuclease activity (F)	PMID:22581770	ECO:0000314 direct assay evidence used in manual assertion	Fig 1	challenge
updatedbyinstructor	HHV8P:MIR2	2013-03-09 12:48:30 CST	GO:0039648 modulation by virus of host protein ubiquitination (P)	PMID:23460925	ECO:0000315 mutant phenotype evidence used in manual assertion	As demonstrated in Figure 7, DC-SIGN and DC-SIGNR are ubiquitylated when in the presence of K5 with an intact RING-CH domain.	challenge
updatedbyinstructor	HHV8P:MIR1	2013-03-09 12:55:02 CST	GO:0039648 modulation by virus of host protein ubiquitination (P)	PMID:23460925	ECO:0000315 mutant phenotype evidence used in manual assertion	As demonstrated in Figure 7, DC-SIGN and DC-SIGNR are ubiquitylated when in the presence of K3 with an intact RING-CH domain.	challenge
updatedbyinstructor	HHV8P:MIR2	2013-03-09 14:19:40 CST	GO:0044078 positive regulation by symbiont of host receptor-mediated endocytosis (P)	PMID:23460925	ECO:0000315 mutant phenotype evidence used in manual assertion	As demonstrated by Figure 4C, in the presence of wildtype K5, a small but significant acceleration in endocytosis of DC-SIGN was detected	challenge

acceptable:3
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Willcoxe

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Acapello, Team Blot Blot Western Baby	PSEPK:TOLB	GO:0009279 - cell outer membrane (C)	PMID:10940016	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 6
updatedbyinstructor	Cwolterman, Team Pinball Wizards	HUMAN:IFNG	GO:0010634 - positive regulation of epithelial cell migration (P)	PMID:11698262	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1 fixed by Willcoxe
acceptable	Bennettv, Team Viral Load Challengers	PEDV7:VEMP	GO:0033664 - obsolete positive regulation by symbiont of host defense-related protein level (P)	PMID:23332027	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3
updatedbyinstructor	Vthompsonui, Team Buster McThunderstick	BACSU:EZRA	GO:0051781 - positive regulation of cell division (P)	PMID:23249255	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1 fixed by Willcoxe

2 annotations fixed by Willcoxe

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