PMID:11222676

Citation	Nogal, ML, González de Buitrago, G, Rodríguez, C, Cubelos, B, Carrascosa, AL, Salas, ML and Revilla, Y (2001) African swine fever virus IAP homologue inhibits caspase activation and promotes cell survival in mammalian cells. J. Virol. 75:2535-43
Abstract	African swine fever virus (ASFV) A224L is a member of the inhibitor of apoptosis protein (IAP) family. We have investigated the antiapoptotic function of the viral IAP both in stably transfected cells and in ASFV-infected cells. A224L was able to substantially inhibit caspase activity and cell death induced by treatment with tumor necrosis factor alpha and cycloheximide or staurosporine when overexpressed in Vero cells by gene transfection. We have also observed that ASFV infection induces caspase activation and apoptosis in Vero cells. Furthermore, using a deletion mutant of ASFV lacking the A224L gene, we have shown that the viral IAP modulates the proteolytic processing of the effector cell death protease caspase-3 and the apoptosis which are induced in the infected cells. Our findings indicate that A224L interacts with the proteolytic fragment of caspase-3 and inhibits the activity of this protease during ASFV infection. These observations could indicate a conserved mechanism of action for ASFV IAP and other IAP family members to suppress apoptosis.
Links	PubMed PMC115875 Online version:10.1128/JVI.75.6.2535-2543.2001
Keywords	African Swine Fever Virus/genetics; African Swine Fever Virus/physiology; Animals; Apoptosis; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Blotting, Western; Caspase 3; Caspases/metabolism; Cell Survival; Cercopithecus aethiops; Enzyme Activation; Flow Cytometry; Gene Deletion; Inhibitor of Apoptosis Proteins; Insect Proteins; Proteins; Transfection; Vero Cells; Viral Structural Proteins/genetics; Viral Structural Proteins/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ASFB7:IAP	involved_in	GO:0039650: suppression by virus of host cysteine-type endopeptidase activity involved in apoptotic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
ASFB7:IAP		GO:0039650: suppression by virus of host cysteine-type endopeptidase activity involved in apoptotic process	ECO:0000314:		P		As seen in Figure 5, viral IAP inhibits host cell apopotosis by modulating caspase-3 activity within the cell. This was determined by comparing the levels of caspase-3 activity in Vero cells infected with the deletion mutant ΔIAP and the parental virus BA71V, using a caspase-specific assay	complete CACAO 6395

References

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