User:Swhipple

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	EBOZM:VP40	2013-02-08 12:48:21 CST	GO:0045121 membrane raft (C)	PMID:14673115	ECO:0000314 direct assay evidence used in manual assertion	Figure 2: This Western blot using anti-VP40 antibody shows lipid rafts extracted from VP40 transfected cells are enriched with VP40 oligomers.	challenge
updatedbyinstructor	EBOZM:VP40	2013-02-08 12:49:39 CST	GO:0046761 viral budding from plasma membrane (P)	PMID:11333902	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3: These Western blots demonstrate the ability of VP40 to produce membrane bound particles in the absence of other viral proteins.	challenge
updatedbyinstructor	EBOZM:VGP	2013-02-08 12:52:11 CST	GO:0045121 membrane raft (C)	PMID:11877482	ECO:0000314 direct assay evidence used in manual assertion	Figure 1: This Western blot for GP demonstrates the localization of GP in the lipid rafts.	challenge
acceptable	EBOZM:VGP	2013-02-08 12:52:11 CST	GO:0046761 viral budding from plasma membrane (P)	PMID:11877482	ECO:0000314 direct assay evidence used in manual assertion	Figure 6B: Immunogold staining of the virus like particles with anti-Ebola GP antibodies demonstrated the identity of the spikes on the surface of the particles as Ebola GP.	challenge
updatedbyinstructor	EBOZM:VP24	2013-02-08 12:56:18 CST	GO:0020002 host cell plasma membrane (C)	PMID:12525613	ECO:0000314 direct assay evidence used in manual assertion	Figure 2: This indirect immunofluorescence analysis demonstrates the localization of VP24 into the plasma membrane.	challenge
acceptable	EBOZM:VP24	2013-02-08 12:56:18 CST	GO:0046761 viral budding from plasma membrane (P)	PMID:12525613	ECO:0000314 direct assay evidence used in manual assertion	Figure 7: This figure shows the results of SDS-PAGE that detected VP24 in the budding virus like particles.	challenge
updatedbyinstructor	EBOZM:VP40	2013-02-09 23:32:20 CST	GO:0044385 integral to membrane of host cell (C)	PMID:11333902	ECO:0000314 direct assay evidence used in manual assertion	Figures 5 and 7: These two Western blots demonstrate the integral membrane characteristic of VP40 using Triton X-114 phase-partitioning analysis and flotation analysis.	challenge
unacceptable	EBOZM:VP40	2013-03-12 21:42:28 CDT	GO:0020002 host cell plasma membrane (C)	PMID:15650213	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4. Indirect immunofluroescence with anti-VP40 monoclonal antibody was used to localize VP40 in small patches in the plasma membrane.	challenge
unacceptable	EBOZM:VP35	2013-03-12 21:58:44 CDT	GO:0003690 double-stranded DNA binding (F)	PMID:16698997	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1. All of these results show VP35s ability to bind dsDNA. An in vitro "pull-down" assay was used. Cell lysates were prepared and incubated with pIC-Sepharose and then visualized by Western blotting.	challenge
updatedbyinstructor	EBOZM:VP35	2013-03-12 22:12:34 CDT	GO:0039501 suppression by virus of host type I interferon production (P)	PMID:16698997	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2. The abilities of wild-type VP35, R312A, and K309A to inhibit the SeV-induced activation of an IFN-Beta promoter reporter plasmid were compared. Western blotting was used to demonstrate the ability of VP35 to regulate IFN-Beta in Figure 2A, while Figure B shows the results from a panel overlay onto Vero cells in a 96-well plate and subsequent fluorescence.	challenge

acceptable:2
unacceptable:2
requires_changes:0
flagged:0

Annotations challenged by Swhipple

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	Jbeasley, Team Everything Burrito	HV1BR:TAT	GO:1901856 - negative regulation of cellular respiration (P)	PMID:17202348	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 4 fixed by Deradeza
updatedbyinstructor	Zstielper, Team Everything Burrito	JSRV:ENV	GO:0016032 - viral process (P)	PMID:16873259	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2 fixed by Swhipple
requireschanges	Acapello, Team Blot Blot Western Baby	NVN68:VP2	GO:0043489 - RNA stabilization (P)	PMID:14557646	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2
updatedbyinstructor	Kathrynmartin, Team M Squared	MOUSE:LHX2	GO:0007411 - axon guidance (P)	PMID:22457488	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1 fixed by Swhipple
updatedbyinstructor	Kathrynmartin, Team M Squared	DANRE:NPHP3	GO:0042384 - cilium assembly (P)	PMID:20462968	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1 fixed by Swhipple

3 annotations fixed by Swhipple

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