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PMID:14673115
| Citation |
Panchal, RG, Ruthel, G, Kenny, TA, Kallstrom, GH, Lane, D, Badie, SS, Li, L, Bavari, S and Aman, MJ (2003) In vivo oligomerization and raft localization of Ebola virus protein VP40 during vesicular budding. Proc. Natl. Acad. Sci. U.S.A. 100:15936-41 |
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| Abstract |
The matrix protein VP40 plays a critical role in Ebola virus assembly and budding, a process that utilizes specialized membrane domains known as lipid rafts. Previous studies with purified protein suggest a role for oligomerization of VP40 in this process. Here, we demonstrate VP40 oligomers in lipid rafts of mammalian cells, virus-like particles, and in the authentic Ebola virus. By mutagenesis, we identify several critical C-terminal sequences that regulate oligomerization at the plasma membrane, association with detergent-resistant membranes, and vesicular release of VP40, directly linking these phenomena. Furthermore, we demonstrate the active recruitment of TSG101 into lipid rafts by VP40. We also report the successful application of the biarsenic fluorophore, FlAsH, combined with a tetracysteine tag for imaging of Ebola VP40 in live cells. |
| Links |
PubMed PMC307671 Online version:10.1073/pnas.2533915100 |
| Keywords |
Cell Line; Cell Membrane/virology; Ebolavirus/genetics; Humans; Kidney; Membrane Microdomains/virology; Microscopy, Confocal; Nucleoproteins/analysis; Nucleoproteins/genetics; Recombinant Proteins/analysis; Transfection; Viral Core Proteins/analysis; Viral Core Proteins/genetics |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0045121: membrane raft |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0045121: membrane raft |
ECO:0000314: |
C |
Figure 2: This Western blot using anti-VP40 antibody shows lipid rafts extracted from VP40 transfected cells are enriched with VP40 oligomers. |
complete | ||||
See also
References
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