EBOZM:VP40

Species (Taxon ID)	Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus). (128952)
Gene Name(s)	VP40
Protein Name(s)	Matrix protein VP40 Membrane-associated protein VP40
External Links
UniProt	Q05128
EMBL	X61274 L11365 AF086833 AF272001 AF499101 AY142960
RefSeq	NP_066245.1
PDB	1H2C 1H2D 2KQ0 4EJE 4LDB 4LDD 4LDI 4LDM
PDBsum	1H2C 1H2D 2KQ0 4EJE 4LDB 4LDD 4LDI 4LDM
ProteinModelPortal	Q05128
SMR	Q05128
MINT	MINT-151825
GeneID	911825
EvolutionaryTrace	Q05128
Proteomes	UP000007209
GO	GO:0033645 GO:0020002 GO:0044385 GO:0045121 GO:0055036 GO:0003723 GO:0039660 GO:0044414 GO:0046761 GO:0039702
InterPro	IPR008986
Pfam	PF07447
PIRSF	PIRSF018327
SUPFAM	SSF50012

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Notes	Status
	GO:0044414	suppression of host defenses	PMID:21228243^[1]	ECO:0000315			P	Fig. 2 Suppression of siRNA	complete CACAO 2418
	GO:0020002	host cell plasma membrane	PMID:21223600^[2]	ECO:0000315			C	Fig. 3A	complete CACAO 2446
	GO:0045121	membrane raft	PMID:14673115^[3]	ECO:0000314			C	Figure 2: This Western blot using anti-VP40 antibody shows lipid rafts extracted from VP40 transfected cells are enriched with VP40 oligomers.	complete CACAO 6398
	GO:0046761	viral budding from plasma membrane	PMID:11333902^[4]	ECO:0000315			P	Figure 3: These Western blots demonstrate the ability of VP40 to produce membrane bound particles in the absence of other viral proteins.	complete CACAO 6399
	GO:0044385	integral to membrane of host cell	PMID:11333902^[4]	ECO:0000314			C	Figures 5 and 7: These two Western blots demonstrate the integral membrane characteristic of VP40 using Triton X-114 phase-partitioning analysis and flotation analysis.	complete CACAO 6498
	GO:0020002	host cell plasma membrane	PMID:15650213^[5]	ECO:0000315			C	Figure 4. Indirect immunofluroescence with anti-VP40 monoclonal antibody was used to localize VP40 in small patches in the plasma membrane.	complete CACAO 6913
	GO:0046788	egress of virus within host cell	PMID:23297401^[6]	ECO:0000315			P	Figure 8	complete CACAO 8554
	GO:0046755	viral budding	PMID:16837071^[7]	ECO:0000315			P	Fig. 4	complete CACAO 8595
	GO:0019076	viral release from host cell	PMID:23297401^[6]	ECO:0000315			P	Figure 2B, 2C show that when certain key amino acids are mutated (L213A, L295A, V298A) they do not induce the same amount of surface pressure into the plasma membrane as compared to WT	complete CACAO 8653
	GO:0044385	integral to membrane of host cell	PMID:23297401^[6]	ECO:0000315			C	Figure 3	complete CACAO 8654
	GO:0019067	viral assembly, maturation, egress, and release	PMID:23663837^[8]	ECO:0000315			P	Fig. 6 showing wtVP40 causes vesicle formation for viral egress in GUVs compared to mutant phenotype L295A.	complete CACAO 8686
	GO:0020002	host cell plasma membrane	PMID:23297401^[6]	ECO:0000315			C	Figure 8: Western blots of cells transfected with WT VP40 and several hydrophobic mutants demonstrate that C-terminal domain plasma membrane penetration is critical for viral egress.	complete CACAO 9138
	GO:0070676	intralumenal vesicle formation	PMID:25315776^[9]	ECO:0000314			P	Figure 2B shows intralumenal vesicle formation in the presence of VP40 compared to the GUV control seen in 2A.	complete CACAO 10859
	GO:1990606	membrane scission GTPase motor activity	PMID:25315776^[9]	ECO:0000314			F	Figures 5B and 5F show scission of buds by VP40 in GUV membranes.	complete CACAO 10860
involved_in	GO:0046761	viral budding from plasma membrane	PMID:23663837^[8]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0044385	integral to membrane of host cell	PMID:23297401^[6]	ECO:0000315	mutant phenotype evidence used in manual assertion		C	Seeded From UniProt	complete
involved_in	GO:0075733	intracellular transport of virus	PMID:23297401^[6]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0046755	viral budding	PMID:16837071^[7]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0046761	viral budding from plasma membrane	PMID:11333902^[4]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0045121	membrane raft	PMID:14673115^[3]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
involved_in	GO:0044414	suppression of host defenses	PMID:21228243^[1]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0044385	integral to membrane of host cell	PMID:11333902^[4]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0033644	host cell membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1043	C	Seeded From UniProt	complete
part_of	GO:0020002	host cell plasma membrane	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1032 UniProtKB-SubCell:SL-0375	C	Seeded From UniProt	complete
part_of	GO:0030430	host cell cytoplasm	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1035 UniProtKB-SubCell:SL-0381	C	Seeded From UniProt	complete
involved_in	GO:0016032	viral process	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0945	P	Seeded From UniProt	complete
part_of	GO:0005576	extracellular region	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0964	C	Seeded From UniProt	complete
enables	GO:0039660	structural constituent of virion	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0468	F	Seeded From UniProt	complete
part_of	GO:0044174	host cell endosome	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1039	C	Seeded From UniProt	complete
involved_in	GO:0039702	viral budding via host ESCRT complex	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1187	P	Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0472	C	Seeded From UniProt	complete
involved_in	GO:0046755	viral budding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1198	P	Seeded From UniProt	complete
part_of	GO:0019012	virion	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0946	C	Seeded From UniProt	complete
enables	GO:0003723	RNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0694	F	Seeded From UniProt	complete
involved_in	GO:0030683	evasion or tolerance by virus of host immune response	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0899	P	Seeded From UniProt	complete
part_of	GO:0033645	host cell endomembrane system	GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-SubCell:SL-0398	C	Seeded From UniProt	complete
part_of	GO:0055036	virion membrane	GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-SubCell:SL-0275	C	Seeded From UniProt	complete
part_of	GO:0044185	host cell late endosome membrane	GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-SubCell:SL-0400	C	Seeded From UniProt	complete
part_of	GO:0070062	extracellular exosome	GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-SubCell:SL-0466	C	Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ ^1.0 ^1.1 Fabozzi, G et al. (2011) Ebolavirus proteins suppress the effects of small interfering RNA by direct interaction with the mammalian RNA interference pathway. J. Virol. 85 2512-23 PubMed GONUTS page
↑ Bhattacharyya, S & Hope, TJ (2011) Full-length Ebola glycoprotein accumulates in the endoplasmic reticulum. Virol. J. 8 11 PubMed GONUTS page
↑ ^3.0 ^3.1 Panchal, RG et al. (2003) In vivo oligomerization and raft localization of Ebola virus protein VP40 during vesicular budding. Proc. Natl. Acad. Sci. U.S.A. 100 15936-41 PubMed GONUTS page
↑ ^4.0 ^4.1 ^4.2 ^4.3 Jasenosky, LD et al. (2001) Ebola virus VP40-induced particle formation and association with the lipid bilayer. J. Virol. 75 5205-14 PubMed GONUTS page
↑ Hoenen, T et al. (2005) VP40 octamers are essential for Ebola virus replication. J. Virol. 79 1898-905 PubMed GONUTS page
↑ ^6.0 ^6.1 ^6.2 ^6.3 ^6.4 ^6.5 Adu-Gyamfi, E et al. (2013) The Ebola virus matrix protein penetrates into the plasma membrane: a key step in viral protein 40 (VP40) oligomerization and viral egress. J. Biol. Chem. 288 5779-89 PubMed GONUTS page
↑ ^7.0 ^7.1 McCarthy, SE et al. (2006) A luciferase-based budding assay for Ebola virus. J. Virol. Methods 137 115-9 PubMed GONUTS page
↑ ^8.0 ^8.1 Soni, SP et al. (2013) The Ebola virus matrix protein deeply penetrates the plasma membrane: an important step in viral egress. Biophys. J. 104 1940-9 PubMed GONUTS page
↑ ^9.0 ^9.1 Soni, SP & Stahelin, RV (2014) The Ebola virus matrix protein VP40 selectively induces vesiculation from phosphatidylserine-enriched membranes. J. Biol. Chem. 289 33590-7 PubMed GONUTS page

[PMID:21228243-1] 1.0 ^1.1 Fabozzi, G et al. (2011) Ebolavirus proteins suppress the effects of small interfering RNA by direct interaction with the mammalian RNA interference pathway. J. Virol. 85 2512-23 PubMed GONUTS page

[PMID:21223600-2] Bhattacharyya, S & Hope, TJ (2011) Full-length Ebola glycoprotein accumulates in the endoplasmic reticulum. Virol. J. 8 11 PubMed GONUTS page

[PMID:14673115-3] 3.0 ^3.1 Panchal, RG et al. (2003) In vivo oligomerization and raft localization of Ebola virus protein VP40 during vesicular budding. Proc. Natl. Acad. Sci. U.S.A. 100 15936-41 PubMed GONUTS page

[PMID:11333902-4] 4.0 ^4.1 ^4.2 ^4.3 Jasenosky, LD et al. (2001) Ebola virus VP40-induced particle formation and association with the lipid bilayer. J. Virol. 75 5205-14 PubMed GONUTS page

[PMID:15650213-5] Hoenen, T et al. (2005) VP40 octamers are essential for Ebola virus replication. J. Virol. 79 1898-905 PubMed GONUTS page

[PMID:23297401-6] 6.0 ^6.1 ^6.2 ^6.3 ^6.4 ^6.5 Adu-Gyamfi, E et al. (2013) The Ebola virus matrix protein penetrates into the plasma membrane: a key step in viral protein 40 (VP40) oligomerization and viral egress. J. Biol. Chem. 288 5779-89 PubMed GONUTS page

[PMID:16837071-7] 7.0 ^7.1 McCarthy, SE et al. (2006) A luciferase-based budding assay for Ebola virus. J. Virol. Methods 137 115-9 PubMed GONUTS page

[PMID:23663837-8] 8.0 ^8.1 Soni, SP et al. (2013) The Ebola virus matrix protein deeply penetrates the plasma membrane: an important step in viral egress. Biophys. J. 104 1940-9 PubMed GONUTS page

[PMID:25315776-9] 9.0 ^9.1 Soni, SP & Stahelin, RV (2014) The Ebola virus matrix protein VP40 selectively induces vesiculation from phosphatidylserine-enriched membranes. J. Biol. Chem. 289 33590-7 PubMed GONUTS page

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EBOZM:VP40

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