GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:25315776
| Citation |
Soni, SP and Stahelin, RV (2014) The Ebola virus matrix protein VP40 selectively induces vesiculation from phosphatidylserine-enriched membranes. J. Biol. Chem. 289:33590-7 |
|---|---|
| Abstract |
Ebola virus is from the Filoviridae family of viruses and is one of the most virulent pathogens known with ∼ 60% clinical fatality. The Ebola virus negative sense RNA genome encodes seven proteins including viral matrix protein 40 (VP40), which is the most abundant protein found in the virions. Within infected cells VP40 localizes at the inner leaflet of the plasma membrane (PM), binds lipids, and regulates formation of new virus particles. Expression of VP40 in mammalian cells is sufficient to form virus-like particles that are nearly indistinguishable from the authentic virions. However, how VP40 interacts with the PM and forms virus-like particles is for the most part unknown. To investigate VP40 lipid specificity in a model of viral egress we employed giant unilamellar vesicles with different lipid compositions. The results demonstrate VP40 selectively induces vesiculation from membranes containing phosphatidylserine (PS) at concentrations of PS that are representative of the PM inner leaflet content. The formation of intraluminal vesicles was not significantly detected in the presence of other important PM lipids including cholesterol and polyvalent phosphoinositides, further demonstrating PS selectivity. Taken together, these studies suggest that PM phosphatidylserine may be an important component of Ebola virus budding and that VP40 may be able to mediate PM scission. |
| Links |
PubMed PMC4246110 Online version:10.1074/jbc.M114.586396 |
| Keywords |
|
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0070676: intralumenal vesicle formation |
ECO:0000314: |
P |
Figure 2B shows intralumenal vesicle formation in the presence of VP40 compared to the GUV control seen in 2A. |
complete | ||||
| GO:1990606: membrane scission GTPase motor activity |
ECO:0000314: |
F |
Figures 5B and 5F show scission of buds by VP40 in GUV membranes. |
complete | ||||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
