User:Jbeasley

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	HV1BR:TAT	2013-02-07 14:40:22 CST	GO:0060081 membrane hyperpolarization (P)	PMID:17202348	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 shows that adding tat protein to cortical neurons caused a 10% loss in rhod123 fluorescence which is a measurement method of mitochondrial membrane potential. This result proves that the tat protein induces mitochondrial hyperpolarization.	challenge
updatedbyinstructor	HV1BR:TAT	2013-02-08 17:53:47 CST	GO:1901856 negative regulation of cellular respiration (P)	PMID:17202348	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 demonstrates the inhibiting effect of the tat protein on mitochondrial complexes III and IV while having no effect on complexes I and II. The ETC activity of an isolated mitochondrial protein was monitored after adding tat protein, and the graph (A) shows the effect that the tat treatment had on each complex. Complexes III and IV show inhibited activity once the tat protein was introduced. (B) shows the concentration dependent inhibition of complex IV activity.	challenge
acceptable	HV1BR:TAT	2013-02-10 12:40:30 CST	GO:0051092 positive regulation of NF-kappaB transcription factor activity (P)	PMID:10400814	ECO:0000314 direct assay evidence used in manual assertion	Figure 3B shows the gel retardation analysis of NF-kappaB complexes induced by Tat in both NIH 3T3 and PKR knockout mouse cells. The analysis reveals that neither the tat protein nor the PKR (a Cellular Interferon-Inducible, Double-Stranded RNA-Dependent Protein Kinase) can activate the NF-kappa-B transcription factor (lanes 1 and 2). When the tat protein and PKR worked together (Lane 3), this produced a positive activation of NF-kappaB. This proves that the tat protein must activate NF-kappaB through PKR.	challenge
updatedbyinstructor	HV1BR:TAT	2013-02-10 13:06:18 CST	GO:0052312 modulation of transcription in other organism involved in symbiotic interaction (P)	PMID:22593154	ECO:0000314 direct assay evidence used in manual assertion	Figure 3A demonstrates that the Tat addition to Jurkat T cells caused a 10.8-fold increase in Human Endogenous Retrovirus Type-K (HML-2) gag RNA production after 6 hours. This concludes that the tat protein activates the expression of HML-2.	challenge
acceptable	HV1BR:TAT	2013-02-10 13:19:46 CST	GO:1900182 positive regulation of protein localization to nucleus (P)	PMID:22219277	ECO:0000314 direct assay evidence used in manual assertion	Figure 1C indicates that the tat protein triggers the ZO-1 protein localization to the nucleus. This is shown by significantly increased ZO-1 expression in the nucleus of adenovirus-infected hCMEC/D3 cells depicted in the bar graph in Figure 1C.	challenge

acceptable:3
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Jbeasley

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	Cwolterman, Team Pinball Wizards	HUMAN:IL4	GO:0010633 - negative regulation of epithelial cell migration (P)	PMID:11698262	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2 fixed by Jbeasley
updatedbyinstructor	Alemus, Team Blot Blot Western Baby	HPV16:VE2	GO:0044163 - host cytoskeleton (C)	PMID:15020762	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2
updatedbyinstructor	Alemus, Team Blot Blot Western Baby	HPV18:VE2	GO:0044163 - host cytoskeleton (C)	PMID:15020762	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 7
updatedbyinstructor	Kthornhill, Team Genetic Ninjas	ECOLI:FHUA	GO:0009279 - cell outer membrane (C)	PMID:2156805	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 6 fixed by Kathrynmartin
unacceptable	Melbyd, Team Legend (wait for it) DARY	ADE06:E1U5P1	GO:0008139 - nuclear localization sequence binding (F)	PMID:15483253	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2

1 annotations fixed by Jbeasley

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