PMID:11877482

Citation	Bavari, S, Bosio, CM, Wiegand, E, Ruthel, G, Will, AB, Geisbert, TW, Hevey, M, Schmaljohn, C, Schmaljohn, A and Aman, MJ (2002) Lipid raft microdomains: a gateway for compartmentalized trafficking of Ebola and Marburg viruses. J. Exp. Med. 195:593-602
Abstract	Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and budding. Filoviruses released from infected cells incorporated raft-associated molecules, suggesting that viral exit occurs at the rafts. Ectopic expression of Ebola matrix protein and glycoprotein supported raft-dependent release of filamentous, virus-like particles (VLPs), strikingly similar to live virus as revealed by electron microscopy. Our findings also revealed that the entry of filoviruses requires functional rafts, identifying rafts as the site of virus attack. The identification of rafts as the gateway for the entry and exit of filoviruses and raft-dependent generation of VLPs have important implications for development of therapeutics and vaccination strategies against infections with Ebola and Marburg viruses.
Links	PubMed PMC2193767
Keywords	Cells, Cultured; Ebolavirus/physiology; G(M1) Ganglioside/metabolism; Humans; Marburgvirus/physiology; Membrane Glycoproteins/metabolism; Membrane Microdomains/virology; Viral Envelope Proteins/metabolism; Viral Matrix Proteins/metabolism; Virion/physiology; Virus Assembly
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
EBOZM:VGP	part_of	GO:0045121: membrane raft	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
EBOZM:VGP	involved_in	GO:0046761: viral budding from plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
EBOZM:VGP		GO:0045121: membrane raft	ECO:0000314:		C		Figure 1: This Western blot for GP demonstrates the localization of GP in the lipid rafts.	complete CACAO 6400
EBOZM:VGP		GO:0046761: viral budding from plasma membrane	ECO:0000314:		P		Figure 6B: Immunogold staining of the virus like particles with anti-Ebola GP antibodies demonstrated the identity of the spikes on the surface of the particles as Ebola GP.	complete CACAO 6401

References

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