User:Reinha81

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	ECOLI:RPOH	2013-03-28 14:56:20 CDT	GO:0016987 transcription factor activity, core RNA polymerase binding (F)	PMID:6380765	ECO:0000314 direct assay evidence used in manual assertion	Figure four shows that the core RNA polymerase by itself cannot transcribe from the heat shock promoter without HtpR. However, when just HtpR is added to the core RNA polymerase it can transcribe from the heat shock promoter, indicating that HtpR is a sigma factor and binds RNA polymerase and effects its transcription activity.	challenge
acceptable	SYNP2:RECA	2013-03-28 15:38:54 CDT	GO:0006310 DNA recombination (P)	PMID:3108239	ECO:0000316 genetic interaction evidence used in manual assertion	E.coli with a deleted recA and one lacZ gene on a chromosome and one lacZ gene on a plasmid were used. The lacZ genes being on different alleles caused the E.coli phenotype to be Lac-. When a plasmid containing Synechococcus sp. strain PCC 7002's recA gene were introduced into E.coli some E.coli became Lac+ showing that the synechococcus recA gene functions in DNA recombination. These results are shown in Table 2.	challenge
updatedbyinstructor	ECOLI:RPOH	2013-04-07 18:21:12 CDT	GO:0006351 transcription, DNA-dependent (P)	PMID:6380765	ECO:0000314 direct assay evidence used in manual assertion	Figure 4B shows that HtpR was needed to successfully transcribe from the P1 and P2 promoter of DnaK. DNA was required for this transcription, therefore showing that HtpR is involved in DNA-dependent transcription.	challenge
acceptable	9PARA:Q83639	2013-04-07 18:24:24 CDT	GO:0000768 syncytium formation by plasma membrane fusion (P)	PMID:18287234	ECO:0000315 mutant phenotype evidence used in manual assertion	Different substitutions in the residues of the H protein are shown in figure 5A. While the wild type and some mutant H proteins produced syncytia, some mutants failed to produce syncytia, showing that these residues are important for the function of the H protein, which is to perform syncytia by cell fusion. Figure 4 also shows the cell-to-cell fusion properties of the H protein.	challenge
acceptable	NPVAC:VP35	2013-04-07 18:25:16 CDT	GO:0043066 negative regulation of apoptotic process (P)	PMID:1962198	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 shows that cells containing mutant p35 undergo DNA fragmentation, suggesting apoptosis. However, wild type cells with an intact p35 did not undergo apoptosis as the DNA did not fragmentize. Figure 2 also shows apoptotic characteristics such as blebbing for the vAcAnh mutant. This mutant maps to the p35 gene.	challenge
correctedthroughchallenges	TOBAC:Q9LW91	2013-04-08 19:26:10 CDT	GO:0004089 carbonate dehydratase activity (F)	PMID:12226395	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 shows that control tobacco plants had a 100% change of C180160 values, while tobacco plants with mutants that produced significantly lower levels of carbonic anhydrase only had a 20% change. Therefore carbonic anhydrase must be involved in the carbonate dehydratase activity.	challenge
unacceptable	LISMO:TACY	2013-04-08 19:41:49 CDT	GO:0019836 hemolysis by symbiont of host erythrocytes (P)	PMID:3110067	ECO:0000314 direct assay evidence used in manual assertion	Table 2 shows that mice injected with LLO died due to hemolysis. However, when cholesterol, heat, or N-ethylmaleimide blocked the LLO activity the mice lived.	challenge
requireschanges	HUMAN:RS7	2013-04-10 13:00:14 CDT	GO:1901976 regulation of cell cycle checkpoint (P)	PMID:23563151	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 shows that overexpression of the ribosomal protein S7 resulted in overproduced expression of GADD45alpha, a known cell checkpoint protein. Therefore the S7 ribosomal protein helps to regulate cell cycle checkpoints.	challenge
acceptable	HUMAN:GA45A	2013-04-10 13:16:22 CDT	GO:0071850 mitotic cell cycle arrest (P)	PMID:10097101	ECO:0000314 direct assay evidence used in manual assertion	Figure one shows that Gadd-45 arrests human fibroblasts in the G2/M cell cycle phase.	challenge
acceptable	CANAX:RSR1	2013-04-11 08:30:35 CDT	GO:2000114 regulation of establishment of cell polarity (P)	PMID:23223038	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2 shows that mature hyphae lacking Rsr1 had an Spk intensity index that was much less than the wild type index. Spk is known to be needed for hyphal morphogenesis. Mature hyphae lacking Rsr1 had lower Spk intensity than germ tubes lacking Rsr1, showing that Spk decreases over time without Rsr1. Figure 3 shows strains lacking Rsr1 had decreased localization of Mlc1- YFP in the tips when compared to the wild type. Figure 4A shows the overall defects in morphogenesis of organisms lacking Rsr1 compared to the wild type, including wider cells. Figure 5 shows that Rsr1 focuses Cdc42 activity at the tips of germ tubes and is required to maintain polarized growth needed for development. All of these figures show that Rsr1 interacts and regulates Spk vesicles and its components leading to the establishment of cell polarity.	challenge
acceptable	ACACA:G8IIS4	2013-04-11 11:31:07 CDT	GO:0030414 peptidase inhibitor activity (F)	PMID:23397569	ECO:0000314 direct assay evidence used in manual assertion	Figure 1B and 1C show that the cysteine protease inhibitor inhibited human cathepsin L, human cathepsin B, and papain enzymes. All of these enzymes are part of the C1 family of cysteine proteinases, therefore showing that the cysteine protease inhibitor inhibits protease, or peptidase, activity.	challenge
updatedbyinstructor	ACACA:G8IIS4	2013-04-11 11:39:01 CDT	GO:0005764 lysosome (C)	PMID:23397569	ECO:0000314 direct assay evidence used in manual assertion	Figure two shows that the cysteine protease inhibitor, originally located in the cytoplasm, localizes to the lysosome during cyst formation.	challenge
acceptable	CANAL:HIR1	2013-04-14 13:08:32 CDT	GO:0007059 chromosome segregation (P)	PMID:23417560	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2B shows that cells with a mutant HIR1 gene produced more 4N cells than the wild type. Figure 2C shows these 4N cells were due to abnormal nuclear localization, or an abnormality in chromosome segregation. This indicates that HIR1 is needed for proper chromosome segregation.	challenge
updatedbyinstructor	CANAL:A0A1D8PNS7	2013-04-14 13:24:55 CDT	GO:0007059 chromosome segregation (P)	PMID:23417560	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2B shows that cells with a mutant CAC2 gene produced more 4N cells than the wild type. Figure 2C shows these 4N cells were due to abnormal nuclear localization, or an abnormality in chromosome segregation. This indicates that CAC2 is needed for proper chromosome segregation.	challenge

acceptable:7
unacceptable:1
requires_changes:1
flagged:0

Annotations challenged by Reinha81

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Singhpr4, Team The Monstars	9GAMM:C8YX85	GO:1902087 - dimethylsulfoniopropionate catabolic process (P)	PMID:19807777	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3
correctedthroughchallenges	Singhpr4, Team The Monstars	9SPHN:G0ZNC1	GO:0052764 - exo-oligoalginate lyase activity (F)	other:www.sciencedirect.com/science/article/pii/S1226086X11001651	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2 fixed by Reinha81
updatedbyinstructor	Elizabeth.bajczyk, Team NDSUstuds	MYCTO:L7N5I3	GO:0090143 - obsolete nucleoid organization (P)	PMID:23396914	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 3 fixed by Reinha81

2 annotations fixed by Reinha81

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