ECOLI:RPOH

Species (Taxon ID)	Escherichia coli (strain K12). (83333)
Gene Name(s)	rpoH (ECO:0000255 with HAMAP-Rule:MF_00961) (synonyms: fam, hin, htpR)
Protein Name(s)	RNA polymerase sigma factor RpoH (ECO:0000255 with HAMAP-Rule:MF_00961) Heat shock regulatory protein F33.4 RNA polymerase sigma-32 factor (ECO:0000255 with HAMAP-Rule:MF_00961)
External Links
UniProt	P0AGB3
EMBL	M20668 J05516 K02177 U00039 U00096 AP009048
PIR	H65142
RefSeq	NP_417918.1 YP_491973.1
ProteinModelPortal	P0AGB3
SMR	P0AGB3
DIP	DIP-46203N
IntAct	P0AGB3
STRING	511145.b3461
PaxDb	P0AGB3
PRIDE	P0AGB3
DNASU	947970
EnsemblBacteria	AAC76486 BAE77832
GeneID	12933503 947970
KEGG	ecj:Y75_p3717 eco:b3461
PATRIC	32122366
EchoBASE	EB0890
EcoGene	EG10897
eggNOG	COG0568
HOGENOM	HOG000270269
InParanoid	P0AGB3
KO	K03089
OMA	LNNDEVH
OrthoDB	EOG6D5G0W
PhylomeDB	P0AGB3
BioCyc	EcoCyc:RPOH-MONOMER ECOL316407:JW3426-MONOMER MetaCyc:RPOH-MONOMER
PRO	PR:P0AGB3
Proteomes	UP000000318 UP000000625
Genevestigator	P0AGB3
GO	GO:0005829 GO:0005886 GO:0000990 GO:0003677 GO:0003700 GO:0016987 GO:0008270 GO:0006355 GO:0009408 GO:0001123 GO:0006351
Gene3D	1.10.10.10
HAMAP	MF_00961
InterPro	IPR014284 IPR000943 IPR007627 IPR007630 IPR013325 IPR013324 IPR012759 IPR011991
Pfam	PF04542 PF04545
PRINTS	PR00046
SUPFAM	SSF88659 SSF88946
TIGRFAMs	TIGR02392 TIGR02937
PROSITE	PS00715 PS00716

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Notes	Status
	GO:0033554	cellular response to stress	PMID:1429436^[1]	ECO:0000315			P	figure 4: Beta-galactosidase activity increased six times higher in htpR+ cells harboring TRZ plasmid (luxI,luxR, and lacZ). This demonstrates HtpR and its stimulation of LuxR protein synthesis	complete CACAO 8718
	GO:0033554	cellular response to stress	PMID:1429436^[1]	ECO:0000315			P	figure 5 presents a typical strong induction of the lux system by added concentrations of a synthetic inducer from Vibrio fischeri. Early induction of the lux system by low concentrations of inducer occurs only when LuxR protein is present and active.	complete CACAO 8719
	GO:0033554	cellular response to stress	PMID:1429436^[1]	ECO:0000314			P	figure 6 shows wild-type cells in stationary phase respond immediately to presence of inducer, whereas wild-type cells of other logarithmic growth phases do not.	complete CACAO 8720
	GO:0033554	cellular response to stress	PMID:1429436^[1]	ECO:0000315			P	figure 7 shows that addition of LuxR protein advances luminescence induction of htpR+ cells but has no affect on htpR mutant cells.	complete CACAO 8721
	GO:0033554	cellular response to stress	PMID:1429436^[1]	ECO:0000315			P	figure 8: transforming groESL-containing plasmids into htpR mutant cells showed luminescence induction at a much earlier growth stage than wild-type cells without the plasmid. Luciferase activity of htpR mutant cells was fully recovered by presence of GroESL proteins.	complete CACAO 8722
enables	GO:0000985	bacterial-type RNA polymerase core promoter sequence-specific DNA binding	PMID:9362499^[2]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0001121	bacterial transcription	PMID:9362499^[2]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:9362499^[2]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0006310	DNA recombination	PMID:9362499^[2]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0009009	site-specific recombinase activity	PMID:9362499^[2]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
part_of	GO:0031421	invertasome	PMID:9362499^[2]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
involved_in	GO:0006351	transcription, DNA-templated	PMID:6380765^[3]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0016987	sigma factor activity	PMID:21873635^[4]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10897 PANTHER:PTN001251741	F	Seeded From UniProt	complete
involved_in	GO:0006355	regulation of transcription, DNA-templated	PMID:21873635^[4]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10897 PANTHER:PTN001251741	P	Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	PMID:21873635^[4]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10897 PANTHER:PTN001251741	C	Seeded From UniProt	complete
part_of	GO:0005829	cytosol	PMID:21873635^[4]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10897 PANTHER:PTN001251741	C	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	PMID:21873635^[4]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10897 PANTHER:PTN001251741	F	Seeded From UniProt	complete
enables	GO:0016987	sigma factor activity	PMID:6380765^[3]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0006355	regulation of transcription, DNA-templated	PMID:15757896^[5]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	PMID:15757896^[5]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0016987	sigma factor activity	PMID:6380765^[3]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0010468	regulation of gene expression	PMID:7038687^[6]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0009408	response to heat	PMID:6387714^[7]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0009408	response to heat	PMID:7038687^[6]	ECO:0000270	expression pattern evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0009408	response to heat	PMID:6380765^[3]	ECO:0000270	expression pattern evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0009408	response to heat	PMID:3306410^[8]	ECO:0000270	expression pattern evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	PMID:24358019^[9]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0005829	cytosol	PMID:24358019^[9]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
involved_in	GO:0001121	bacterial transcription	PMID:6380765^[3]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0001121	bacterial transcription	PMID:1565647^[10]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0001121	bacterial transcription	PMID:3887408^[11]	ECO:0000255	match to sequence model evidence used in manual assertion		P	Seeded From UniProt	Missing: with/from
involved_in	GO:0045893	positive regulation of transcription, DNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0001216	P	Seeded From UniProt	complete
involved_in	GO:2000142	regulation of DNA-templated transcription, initiation	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0016987	P	Seeded From UniProt	complete
involved_in	GO:2000142	regulation of DNA-templated transcription, initiation	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0016987	P	Seeded From UniProt	complete
involved_in	GO:2000142	regulation of DNA-templated transcription, initiation	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0016987	P	Seeded From UniProt	complete
involved_in	GO:2000142	regulation of DNA-templated transcription, initiation	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0016987	P	Seeded From UniProt	complete
involved_in	GO:2000142	regulation of DNA-templated transcription, initiation	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0016987	P	Seeded From UniProt	complete
involved_in	GO:2000142	regulation of DNA-templated transcription, initiation	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0016987	P	Seeded From UniProt	complete
enables	GO:0003700	DNA-binding transcription factor activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR000943 InterPro:IPR007627 InterPro:IPR007630 InterPro:IPR012759 InterPro:IPR013325 InterPro:IPR014284	F	Seeded From UniProt	complete
	GO:0016987	transcription factor activity, core RNA polymerase binding	PMID:6380765^[3]	ECO:0000314			F	Figure four shows that the core RNA polymerase by itself cannot transcribe from the heat shock promoter without HtpR. However, when just HtpR is added to the core RNA polymerase it can transcribe from the heat shock promoter, indicating that HtpR is a sigma factor and binds RNA polymerase and effects its transcription activity.	complete CACAO 7146
involved_in	GO:0006352	DNA-templated transcription, initiation	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR000943 InterPro:IPR007627 InterPro:IPR007630 InterPro:IPR012759 InterPro:IPR013325 InterPro:IPR014284	P	Seeded From UniProt	complete
	GO:0006351	transcription, DNA-dependent	PMID:6380765^[3]	ECO:0000314			P	Figure 4B shows that HtpR was needed to successfully transcribe from the P1 and P2 promoter of DnaK. DNA was required for this transcription, therefore showing that HtpR is involved in DNA-dependent transcription.	complete CACAO 7333
involved_in	GO:0006355	regulation of transcription, DNA-templated	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR000943 InterPro:IPR007627 InterPro:IPR007630 InterPro:IPR012759 InterPro:IPR013325 InterPro:IPR014284	P	Seeded From UniProt	complete
enables	GO:0016987	sigma factor activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR012759	F	Seeded From UniProt	complete
enables	GO:0016987	sigma factor activity	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000110761	F	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000110761	F	Seeded From UniProt	complete
part_of	GO:0005737	cytoplasm	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000110761	C	Seeded From UniProt	complete
involved_in	GO:0009408	response to heat	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000110761	P	Seeded From UniProt	complete
involved_in	GO:0010468	regulation of gene expression	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000110761	P	Seeded From UniProt	complete
involved_in	GO:0001123	transcription initiation from bacterial-type RNA polymerase promoter	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000110761	P	Seeded From UniProt	complete
enables	GO:0016987	sigma factor activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0731	F	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0238	F	Seeded From UniProt	complete
involved_in	GO:0006355	regulation of transcription, DNA-templated	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0731	P	Seeded From UniProt	complete
part_of	GO:0005737	cytoplasm	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0963 UniProtKB-SubCell:SL-0086	C	Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 Adar, YY et al. (1992) Formation of the LuxR protein in the Vibrio fischeri lux system is controlled by HtpR through the GroESL proteins. J. Bacteriol. 174 7138-43 PubMed GONUTS page
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 Safo, MK et al. (1997) The transactivation region of the fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms. EMBO J. 16 6860-73 PubMed GONUTS page
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 Grossman, AD et al. (1984) The htpR gene product of E. coli is a sigma factor for heat-shock promoters. Cell 38 383-90 PubMed GONUTS page
↑ ^4.0 ^4.1 ^4.2 ^4.3 ^4.4 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
↑ ^5.0 ^5.1 Zhao, K et al. (2005) The global transcriptional response of Escherichia coli to induced sigma 32 protein involves sigma 32 regulon activation followed by inactivation and degradation of sigma 32 in vivo. J. Biol. Chem. 280 17758-68 PubMed GONUTS page
↑ ^6.0 ^6.1 Yamamori, T & Yura, T (1982) Genetic control of heat-shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K-12. Proc. Natl. Acad. Sci. U.S.A. 79 860-4 PubMed GONUTS page
↑ Yura, T et al. (1984) Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature. Proc. Natl. Acad. Sci. U.S.A. 81 6803-7 PubMed GONUTS page
↑ Straus, DB et al. () The heat shock response of E. coli is regulated by changes in the concentration of sigma 32. Nature 329 348-51 PubMed GONUTS page
↑ ^9.0 ^9.1 Lim, B et al. (2013) Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli. PLoS Biol. 11 e1001735 PubMed GONUTS page
↑ Liberek, K et al. (1992) The DnaK chaperone modulates the heat shock response of Escherichia coli by binding to the sigma 32 transcription factor. Proc. Natl. Acad. Sci. U.S.A. 89 3516-20 PubMed GONUTS page
↑ Cowing, DW et al. (1985) Consensus sequence for Escherichia coli heat shock gene promoters. Proc. Natl. Acad. Sci. U.S.A. 82 2679-83 PubMed GONUTS page

[PMID:1429436-1] 1.0 ^1.1 ^1.2 ^1.3 ^1.4 Adar, YY et al. (1992) Formation of the LuxR protein in the Vibrio fischeri lux system is controlled by HtpR through the GroESL proteins. J. Bacteriol. 174 7138-43 PubMed GONUTS page

[PMID:9362499-2] 2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 Safo, MK et al. (1997) The transactivation region of the fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms. EMBO J. 16 6860-73 PubMed GONUTS page

[PMID:6380765-3] 3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 Grossman, AD et al. (1984) The htpR gene product of E. coli is a sigma factor for heat-shock promoters. Cell 38 383-90 PubMed GONUTS page

[PMID:21873635-4] 4.0 ^4.1 ^4.2 ^4.3 ^4.4 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page

[PMID:15757896-5] 5.0 ^5.1 Zhao, K et al. (2005) The global transcriptional response of Escherichia coli to induced sigma 32 protein involves sigma 32 regulon activation followed by inactivation and degradation of sigma 32 in vivo. J. Biol. Chem. 280 17758-68 PubMed GONUTS page

[PMID:7038687-6] 6.0 ^6.1 Yamamori, T & Yura, T (1982) Genetic control of heat-shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K-12. Proc. Natl. Acad. Sci. U.S.A. 79 860-4 PubMed GONUTS page

[PMID:6387714-7] Yura, T et al. (1984) Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature. Proc. Natl. Acad. Sci. U.S.A. 81 6803-7 PubMed GONUTS page

[PMID:3306410-8] Straus, DB et al. () The heat shock response of E. coli is regulated by changes in the concentration of sigma 32. Nature 329 348-51 PubMed GONUTS page

[PMID:24358019-9] 9.0 ^9.1 Lim, B et al. (2013) Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli. PLoS Biol. 11 e1001735 PubMed GONUTS page

[PMID:1565647-10] Liberek, K et al. (1992) The DnaK chaperone modulates the heat shock response of Escherichia coli by binding to the sigma 32 transcription factor. Proc. Natl. Acad. Sci. U.S.A. 89 3516-20 PubMed GONUTS page

[PMID:3887408-11] Cowing, DW et al. (1985) Consensus sequence for Escherichia coli heat shock gene promoters. Proc. Natl. Acad. Sci. U.S.A. 82 2679-83 PubMed GONUTS page

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ECOLI:RPOH

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