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PMID:1962198

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Citation

Clem, RJ, Fechheimer, M and Miller, LK (1991) Prevention of apoptosis by a baculovirus gene during infection of insect cells. Science 254:1388-90

Abstract

Programmed cell death is an active process of self destruction that is important in both the development and maintenance of multicellular animals. The molecular mechanisms controlling activation or suppression of programmed cell death are largely unknown. Apoptosis, a morphologically and biochemically defined type of programmed cell death commonly seen in vertebrates, was found to be initiated during baculovirus replication in insect cells. A specific viral gene product, p35, was identified as being responsible for blocking the apoptotic response. Identification of the function of this gene will allow further definition of the molecular pathways involved in the regulation of programmed cell death and may identify the role of apoptosis in invertebrate viral defense systems.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Baculoviridae/genetics; Base Sequence; Cell Death; Cell Line; Genes, Viral; Insects; Molecular Sequence Data; Mutagenesis, Insertional; Open Reading Frames; Phenotype; Restriction Mapping

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

NPVAC:VP35

GO:0043066: negative regulation of apoptotic process

ECO:0000315:

P

Figure 3 shows that cells containing mutant p35 undergo DNA fragmentation, suggesting apoptosis. However, wild type cells with an intact p35 did not undergo apoptosis as the DNA did not fragmentize. Figure 2 also shows apoptotic characteristics such as blebbing for the vAcAnh mutant. This mutant maps to the p35 gene.

complete
CACAO 7338

NPVAC:VP35

involved_in

GO:0043066: negative regulation of apoptotic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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