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PMID:23396914

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Citation

Ghosh, S, Indi, SS and Nagaraja, V (2013) Regulation of Lipid Biosynthesis, Sliding Motility, and Biofilm Formation by a Membrane-Anchored Nucleoid-Associated Protein of Mycobacterium tuberculosis. J. Bacteriol. 195:1769-78

Abstract

Bacteria use a number of small basic proteins for organization and compaction of their genomes. By their interaction with DNA, these nucleoid-associated proteins (NAPs) also influence gene expression. Rv3852, a NAP of Mycobacterium tuberculosis, is conserved among the pathogenic and slow-growing species of mycobacteria. Here, we show that the protein predominantly localizes in the cell membrane and that the carboxy-terminal region with the propensity to form a transmembrane helix is necessary for its membrane localization. The protein is involved in genome organization, and its ectopic expression in Mycobacterium smegmatis resulted in altered nucleoid morphology, defects in biofilm formation, sliding motility, and change in apolar lipid profile. We demonstrate its crucial role in regulating the expression of KasA, KasB, and GroEL1 proteins, which are in turn involved in controlling the surface phenotypes in mycobacteria.

Links

PubMed Online version:10.1128/JB.02081-12

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MYCTU:P96225

GO:0046467: membrane lipid biosynthetic process

IMP: Inferred from Mutant Phenotype:

P

Fig 6

complete
CACAO 7363


MYCTU:P96225

GO:2000145: regulation of cell motility

IMP: Inferred from Mutant Phenotype:

P

Fig 5

complete
CACAO 7364


MYCTU:P96225

GO:0042710: biofilm formation

IMP: Inferred from Mutant Phenotype:

P

Fig 5

complete
CACAO 7365


MYCTU:P96225

GO:0005887: integral to plasma membrane

IMP: Inferred from Mutant Phenotype:

C

Fig. 4

complete
CACAO 7366


MYCTU:LSR2

GO:0003677: DNA binding

ECO:0000314:

F

The data from the Figures represents the influence of gene expression.

complete
CACAO 10602

MYCTO:L7N5I3

GO:0090143: nucleoid organization

ECO:0000315:

P

Fig. 2

complete
CACAO 7367

MYCTO:L7N5I3

involved_in

GO:0090143: nucleoid organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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