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HUMAN:GA45A
Contents
| Species (Taxon ID) | Homo sapiens (Human). (9606) | |
| Gene Name(s) | GADD45A (synonyms: DDIT1, GADD45) | |
| Protein Name(s) | Growth arrest and DNA damage-inducible protein GADD45 alpha
DNA damage-inducible transcript 1 protein DDIT-1 | |
| External Links | ||
| UniProt | P24522 | |
| EMBL | L24498 M60974 AY135686 DQ008445 AK314991 AL136120 AL136120 CH471059 CH471059 BC011757 | |
| CCDS | CCDS55605.1 CCDS640.1 | |
| PIR | A39617 | |
| RefSeq | NP_001186670.1 NP_001186671.1 NP_001915.1 | |
| UniGene | Hs.80409 | |
| PDB | 2KG4 | |
| PDBsum | 2KG4 | |
| DisProt | DP00704 | |
| ProteinModelPortal | P24522 | |
| SMR | P24522 | |
| BioGrid | 108014 | |
| DIP | DIP-24217N | |
| IntAct | P24522 | |
| MINT | MINT-133779 | |
| STRING | 9606.ENSP00000360025 | |
| PhosphoSite | P24522 | |
| DMDM | 120751 | |
| PaxDb | P24522 | |
| PRIDE | P24522 | |
| DNASU | 1647 | |
| Ensembl | ENST00000370985 ENST00000370986 | |
| GeneID | 1647 | |
| KEGG | hsa:1647 | |
| UCSC | uc001ddz.2 uc009wbb.2 | |
| CTD | 1647 | |
| GeneCards | GC01P068111 | |
| HGNC | HGNC:4095 | |
| MIM | 126335 | |
| neXtProt | NX_P24522 | |
| PharmGKB | PA28510 | |
| eggNOG | NOG75953 | |
| GeneTree | ENSGT00390000016506 | |
| HOGENOM | HOG000013221 | |
| HOVERGEN | HBG051684 | |
| InParanoid | P24522 | |
| KO | K04402 | |
| OMA | NPHASQW | |
| OrthoDB | EOG7J1819 | |
| PhylomeDB | P24522 | |
| TreeFam | TF300196 | |
| ChiTaRS | GADD45A | |
| EvolutionaryTrace | P24522 | |
| GeneWiki | GADD45A | |
| GenomeRNAi | 1647 | |
| NextBio | 6786 | |
| PRO | PR:P24522 | |
| Proteomes | UP000005640 | |
| Bgee | P24522 | |
| CleanEx | HS_GADD45A | |
| ExpressionAtlas | P24522 | |
| Genevestigator | P24522 | |
| GO | GO:0005737 GO:0005634 GO:0001047 GO:0000185 GO:0006915 GO:0007050 GO:0071479 GO:0071260 GO:0007098 GO:0006281 GO:0006469 GO:0043065 GO:0046330 GO:1900745 GO:2000379 GO:0000079 GO:0042770 | |
| InterPro | IPR024824 IPR029064 IPR004038 | |
| PANTHER | PTHR10411 | |
| Pfam | PF01248 | |
| SUPFAM | SSF55315 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0071850 |
mitotic cell cycle arrest |
ECO:0000314 |
P |
Figure one shows that Gadd-45 arrests human fibroblasts in the G2/M cell cycle phase. |
complete | |||||
| GO:0043537 |
negative regulation of blood vessel endothelial cell migration |
ECO:0000315 |
P |
Figure 3 (d) (in vitro) shows that knockdown of Gadd54a results in blood vessel endothelial cell migration. Therefore, the presence of Gadd54a inhibits this process; it negatively regulates endothelial cell migration. |
complete | |||||
| GO:1903262 |
negative regulation of serine phosphorylation of STAT3 protein |
ECO:0000315 |
P |
Figure 6 b and c show that Gadd45a knockdown in human cells (HeLa) results in enhanced transcriptional activity of STAT3 by increasing the STAT3 phosphorylation site on a serine residue. |
complete | |||||
| GO:0000122 |
negative regulation of transcription from RNA polymerase II promoter |
ECO:0000315 |
P |
Figure 6H shows that absence of Gadd45a results in increased transcriptional activity of STAT3 because of phosphorylation of STAT3 on a Serine residue. Therefore, Gad45a is involved in a process that prevents transcription from an RNA pol II promoter. RNA pol II is the protein that transcribes mRNA in eukaryotes. |
complete | |||||
| GO:0071901 |
negative regulation of protein serine/threonine kinase activity |
ECO:0000315 |
P |
Figure 6B,C shows that Gadd45a is able to prevent the phosphorylation of STAT3 at Ser-727. This means that Gadd45a inhibits the activity of a serine/threonin-specific protein kinase |
complete | |||||
|
enables |
GO:0047485 |
protein N-terminus binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
has_direct_input:(UniProtKB:O14965) |
Seeded From UniProt |
complete | ||
|
enables |
GO:0046982 |
protein heterodimerization activity |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042803 |
protein homodimerization activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0019900 |
kinase binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0071850 |
mitotic cell cycle arrest |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:2000379 |
positive regulation of reactive oxygen species metabolic process |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0071479 |
cellular response to ionizing radiation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0042770 |
signal transduction in response to DNA damage |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:1900745 |
positive regulation of p38MAPK cascade |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0071260 |
cellular response to mechanical stimulus |
ECO:0000270 |
expression pattern evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0046330 |
positive regulation of JNK cascade |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0043065 |
positive regulation of apoptotic process |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000185 |
activation of MAPKKK activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
has_direct_input:(UniProtKB:Q9Y6R4) |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0051726 |
regulation of cell cycle |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
MGI:MGI:107776 |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000823659 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0033153 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0000185 |
activation of MAPKKK activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000823659 |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0016607 |
nuclear speck |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051726 |
regulation of cell cycle |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:P48316 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0007098 |
centrosome cycle |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:P48316 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0006469 |
negative regulation of protein kinase activity |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:P48316 |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:P48316 |
C |
Seeded From UniProt |
complete | ||
|
enables |
GO:0000979 |
RNA polymerase II core promoter sequence-specific DNA binding |
ECO:0000265 |
sequence orthology evidence used in automatic assertion |
UniProtKB:P48316 |
F |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051726 |
regulation of cell cycle |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007050 |
cell cycle arrest |
ECO:0000304 |
author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006915 |
apoptotic process |
ECO:0000304 |
author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006281 |
DNA repair |
ECO:0000304 |
author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000079 |
regulation of cyclin-dependent protein serine/threonine kinase activity |
ECO:0000304 |
author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006977 |
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest |
Reactome:R-HSA-6791312 |
ECO:0000304 |
author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005654 |
nucleoplasm |
Reactome:R-NUL-9617912 |
ECO:0000304 |
author statement supported by traceable reference used in manual assertion |
|
C |
Seeded From UniProt |
complete | |
|
part_of |
GO:0005634 |
nucleus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007049 |
cell cycle |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006974 |
cellular response to DNA damage stimulus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007050 |
cell cycle arrest |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Wang, XW et al. (1999) GADD45 induction of a G2/M cell cycle checkpoint. Proc. Natl. Acad. Sci. U.S.A. 96 3706-11 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 2.3 Yang, F et al. (2013) Gadd45a suppresses tumor angiogenesis via inhibition of the mTOR/STAT3 protein pathway. J. Biol. Chem. 288 6552-60 PubMed GONUTS page
- ↑ 3.0 3.1 3.2 3.3 Sánchez, R et al. (2010) Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase. J. Biol. Chem. 285 22196-201 PubMed GONUTS page
- ↑ 4.0 4.1 4.2 Passos, JF et al. (2010) Feedback between p21 and reactive oxygen production is necessary for cell senescence. Mol. Syst. Biol. 6 347 PubMed GONUTS page
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 Takekawa, M & Saito, H (1998) A family of stress-inducible GADD45-like proteins mediate activation of the stress-responsive MTK1/MEKK4 MAPKKK. Cell 95 521-30 PubMed GONUTS page
- ↑ Smith, AJ et al. (2009) Expression of the Bcl-2 protein BAD promotes prostate cancer growth. PLoS ONE 4 e6224 PubMed GONUTS page
- ↑ 7.0 7.1 7.2 7.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
- ↑ 8.0 8.1 Jin, S et al. (2000) The GADD45 inhibition of Cdc2 kinase correlates with GADD45-mediated growth suppression. J. Biol. Chem. 275 16602-8 PubMed GONUTS page
- ↑ Vairapandi, M et al. (2000) Characterization of MyD118, Gadd45, and proliferating cell nuclear antigen (PCNA) interacting domains. PCNA impedes MyD118 AND Gadd45-mediated negative growth control. J. Biol. Chem. 275 16810-9 PubMed GONUTS page
- ↑ Smith, ML et al. (1994) Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen. Science 266 1376-80 PubMed GONUTS page
c
n
- GO:0043537 ! negative regulation of blood vessel endothelial cell migration
- GO:0000122 ! negative regulation of transcription by RNA polymerase II
- GO:1903262 ! negative regulation of serine phosphorylation of STAT3 protein
- GO:0006469 ! negative regulation of protein kinase activity
- GO:0071901 ! negative regulation of protein serine/threonine kinase activity
- GO:0016607 ! nuclear speck
- GO:0005654 ! nucleoplasm
- GO:0005634 ! nucleus
o
p
- GO:0043065 ! positive regulation of apoptotic process
- GO:0046330 ! positive regulation of JNK cascade
- GO:2000379 ! positive regulation of reactive oxygen species metabolic process
- GO:1900745 ! positive regulation of p38MAPK cascade
- Primates
- GO:0046982 ! protein heterodimerization activity
- GO:0042803 ! protein homodimerization activity
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