User:Mkline

CACAO II Spring 2012

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My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	HUMAN:GRP1	2011-10-04 20:00:46 CDT	GO:0043406 positive regulation of MAP kinase activity (P)	PMID:15899849	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 4B. Erk1/Erk2 phosphorylation (via MAPK) was delayed and reduced in RasGRP1- mutants.	challenge
requireschanges	HUMAN:GRP1	2011-10-04 20:21:26 CDT	GO:0010628 positive regulation of gene expression (P)	PMID:21966541	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3	challenge
acceptable	HUMAN:LCP2	2011-10-04 20:49:23 CDT	GO:0045860 positive regulation of protein kinase activity (P)	PMID:14624253	ECO:0000270 expression pattern evidence used in manual assertion	Figure 1, Fig 2BC, Fig 3CE	challenge
acceptable	HUMAN:LAT	2011-10-04 20:51:47 CDT	GO:0045860 positive regulation of protein kinase activity (P)	PMID:14624253	ECO:0000270 expression pattern evidence used in manual assertion	Figure 1, 2BC, and Fig. 3C-E. Figure 3 in particular indicates that "proper localization and signaling through the adapter LAT are required for normal suppression of RAG gene expression".	challenge
acceptable	MOUSE:GDF8	2011-10-18 19:58:19 CDT	GO:0048632 negative regulation of skeletal muscle tissue growth (P)	PMID:9139826	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 4a-d; disruption of GDF-8's normal functioning	challenge
unacceptable	BOVIN:GDF8	2011-10-18 20:11:22 CDT	GO:0048632 negative regulation of skeletal muscle tissue growth (P)	PMID:9314496	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2; expression of non-functional allele in cattle	challenge
acceptable	HUMAN:GDF8	2011-10-18 20:19:48 CDT	GO:0048632 negative regulation of skeletal muscle tissue growth (P)	PMID:15215484	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2; comparison of wild-type versus mutant allele	challenge
acceptable	HUMAN:GRP1	2011-11-01 20:21:46 CDT	GO:0090630 activation of GTPase activity (P)	PMID:12845332	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 3e; overexpression of RasGRP1 was induced to determine the protein's normal function	challenge
acceptable	HUMAN:RASL2	2011-11-01 20:17:00 CDT	GO:0034261 negative regulation of Ras GTPase activity (P)	PMID:12845332	ECO:0000314 direct assay evidence used in manual assertion	Fig 5b; over- and underexpression to determine role of CAPRI; directly blocks Ras activation	challenge
acceptable	HUMAN:RASL2	2011-11-01 20:18:55 CDT	GO:0034261 negative regulation of Ras GTPase activity (P)	PMID:11448776	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 3; mutant and wild-type compared to determine CAPRI's role in Ras activation;	challenge
acceptable	HUMAN:GRP1	2011-11-01 20:33:43 CDT	GO:0090630 activation of GTPase activity (P)	PMID:10807788	ECO:0000314 direct assay evidence used in manual assertion	Fig 4; overexpression of RasGRP1 -> hypersensitive Jurkat cells. However, we observed that RasGRP can activate Ras in vivo even in the presence of EGTA plus BAPTA/AM, compounds that sequester extracellular and intracellular calcium, respectively (data not shown).	challenge
acceptable	MOUSE:GRP1	2011-11-01 20:48:39 CDT	GO:0033089 positive regulation of T cell differentiation in thymus (P)	PMID:11017103	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 2; inhibition of mouse RasGRP1 led to impaired T-cell differentiation	challenge
acceptable	MOUSE:GRP1	2011-11-01 20:56:13 CDT	GO:0090630 activation of GTPase activity (P)	PMID:11017103	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 4; RasGRP inactivation in mutants led to no Ras activation	challenge
acceptable	MOUSE:RAG1	2011-11-15 19:33:40 CST	GO:0045582 positive regulation of T cell differentiation (P)	PMID:1547488	ECO:0000315 mutant phenotype evidence used in manual assertion	Figures 3 and 4: in particular the lack of anti-IgM and -IgD staining indicates that there are no mature B cells in the spleen or bone marrow	challenge
acceptable	MOUSE:RAG2	2011-11-15 19:38:52 CST	GO:0030183 B cell differentiation (P)	PMID:1547487	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 3; there is a pronounced lack of mature B cells in RAG-2 deficient mice	challenge
acceptable	MOUSE:GRP1	2011-11-15 19:50:08 CST	GO:0032825 positive regulation of natural killer cell differentiation (P)	PMID:21957144	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 1D, 1E: RasGRP1 is necessary for early NKC development	challenge
acceptable	MOUSE:GRP1	2011-11-15 20:00:49 CST	GO:0032816 positive regulation of natural killer cell proliferation (P)	PMID:21957144	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 3F: lack of natural killer cell proliferation of RasGRP -/- mutant	challenge
acceptable	MOUSE:CD1D1	2011-11-15 20:44:17 CST	GO:0033084 regulation of immature T cell proliferation in thymus (P)	PMID:11550008	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 1c: DPdull tetramers are derived from CD1d-mediated selection; they were no longer present in 9-day old mice thymi	challenge
acceptable	MOUSE:CD1D1	2011-11-15 20:51:50 CST	GO:0001865 NK T cell differentiation (P)	PMID:9133426	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 3: the number of NT cells in thymus reduced 5-6x in mutant strain	challenge

acceptable:17
unacceptable:1
requires_changes:1
flagged:0