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Category:Team Cheesesteak

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableMOUSE:CD1D1Mkline, Team Cheesesteak2011-11-15 20:51:50 CSTGO:0001865 NK T cell differentiation (P)PMID:9133426ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3: the number of NT cells in thymus reduced 5-6x in mutant strain

challenge
acceptableMOUSE:CD1D1Mkline, Team Cheesesteak2011-11-15 20:44:17 CSTGO:0033084 regulation of immature T cell proliferation in thymus (P)PMID:11550008ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1c: DPdull tetramers are derived from CD1d-mediated selection; they were no longer present in 9-day old mice thymi

challenge
acceptableECOLI:FTNAAfg8920, Team Cheesesteak2011-11-15 20:21:42 CSTGO:0006979 iron-sulfur cluster assembly (P)PMID:18618270ECO:0000314 direct assay evidence used in manual assertion

Fig. 3 shows that FtnA releaves the production of hydroxyl free radicals by scavenging the iron

challenge
acceptableMOUSE:GRP1Mkline, Team Cheesesteak2011-11-15 20:00:49 CSTGO:0032816 positive regulation of natural killer cell proliferation (P)PMID:21957144ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3F: lack of natural killer cell proliferation of RasGRP -/- mutant

challenge
acceptableMOUSE:GRP1Mkline, Team Cheesesteak2011-11-15 19:50:08 CSTGO:0032825 positive regulation of natural killer cell differentiation (P)PMID:21957144ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1D, 1E: RasGRP1 is necessary for early NKC development

challenge
acceptableMOUSE:RAG2Mkline, Team Cheesesteak2011-11-15 19:38:52 CSTGO:0030183 B cell differentiation (P)PMID:1547487ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3; there is a pronounced lack of mature B cells in RAG-2 deficient mice

challenge
acceptableMOUSE:RAG1Mkline, Team Cheesesteak2011-11-15 19:33:40 CSTGO:0045582 positive regulation of T cell differentiation (P)PMID:1547488ECO:0000315 mutant phenotype evidence used in manual assertion

Figures 3 and 4: in particular the lack of anti-IgM and -IgD staining indicates that there are no mature B cells in the spleen or bone marrow

challenge
acceptableMOUSE:GRP1Mkline, Team Cheesesteak2011-11-01 20:56:13 CDTGO:0090630 activation of GTPase activity (P)PMID:11017103ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 4; RasGRP inactivation in mutants led to no Ras activation

challenge
acceptableMOUSE:GRP1Mkline, Team Cheesesteak2011-11-01 20:48:39 CDTGO:0033089 positive regulation of T cell differentiation in thymus (P)PMID:11017103ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 2; inhibition of mouse RasGRP1 led to impaired T-cell differentiation

challenge
acceptableHUMAN:GRP1Mkline, Team Cheesesteak2011-11-01 20:33:43 CDTGO:0090630 activation of GTPase activity (P)PMID:10807788ECO:0000314 direct assay evidence used in manual assertion

Fig 4; overexpression of RasGRP1 -> hypersensitive Jurkat cells. However, we observed that RasGRP can activate Ras in vivo even in the presence of EGTA plus BAPTA/AM, compounds that sequester extracellular and intracellular calcium, respectively (data not shown).

challenge
acceptableHCVJA:POLGAfg8920, Team Cheesesteak2011-11-01 20:22:31 CDTGO:0003724 RNA helicase activity (F)PMID:8970970ECO:0000314 direct assay evidence used in manual assertion

Fig. 3 shows the optimizing reaction conditions for HCV NS3 RNA helicase

challenge
acceptableHUMAN:GRP1Mkline, Team Cheesesteak2011-11-01 20:21:46 CDTGO:0090630 activation of GTPase activity (P)PMID:12845332ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3e; overexpression of RasGRP1 was induced to determine the protein's normal function

challenge
acceptableHUMAN:RASL2Mkline, Team Cheesesteak2011-11-01 20:18:55 CDTGO:0034261 negative regulation of Ras GTPase activity (P)PMID:11448776ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3; mutant and wild-type compared to determine CAPRI's role in Ras activation;

challenge
acceptableHUMAN:RASL2Mkline, Team Cheesesteak2011-11-01 20:17:00 CDTGO:0034261 negative regulation of Ras GTPase activity (P)PMID:12845332ECO:0000314 direct assay evidence used in manual assertion

Fig 5b; over- and underexpression to determine role of CAPRI; directly blocks Ras activation

challenge
acceptableNOCAE:REBFAfg8920, Team Cheesesteak2011-11-01 20:04:54 CDTGO:0042602 flavin reductase activity (F)PMID:15743914ECO:0000314 direct assay evidence used in manual assertion

Addition of RebF provides FADH2 for the halogenase to produce 7-chlorotryptophan during rebeccamycin biosynthesis


challenge
acceptableHUMAN:TGFB1Afg8920, Team Cheesesteak2011-10-18 20:51:45 CDTGO:0045596 negative regulation of cell differentiation (P)PMID:19357233ECO:0000270 expression pattern evidence used in manual assertion

Fig. 1. shows inhibition by myostatin of human skeletal muscle cells (HuSkMC).

challenge
acceptableHUMAN:GDF8Mkline, Team Cheesesteak2011-10-18 20:19:48 CDTGO:0048632 negative regulation of skeletal muscle tissue growth (P)PMID:15215484ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2; comparison of wild-type versus mutant allele

challenge
unacceptableBOVIN:GDF8Mkline, Team Cheesesteak2011-10-18 20:11:22 CDTGO:0048632 negative regulation of skeletal muscle tissue growth (P)PMID:9314496ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2; expression of non-functional allele in cattle

challenge
acceptableMOUSE:GDF8Mkline, Team Cheesesteak2011-10-18 19:58:19 CDTGO:0048632 negative regulation of skeletal muscle tissue growth (P)PMID:9139826ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 4a-d; disruption of GDF-8's normal functioning

challenge
acceptableHUMAN:BACH1Afg8920, Team Cheesesteak2011-10-18 19:26:15 CDTGO:0006281 DNA repair (P)PMID:11301010ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 5 shows that the mutant interferes with BACH1 ability to timely repair double-strand breaks that BRCA1 usually makes.

challenge
acceptableHUMAN:LATMkline, Team Cheesesteak2011-10-04 20:51:47 CDTGO:0045860 positive regulation of protein kinase activity (P)PMID:14624253ECO:0000270 expression pattern evidence used in manual assertion

Figure 1, 2BC, and Fig. 3C-E. Figure 3 in particular indicates that "proper localization and signaling through the adapter LAT are required for normal suppression of RAG gene expression".

challenge
acceptableHUMAN:LCP2Mkline, Team Cheesesteak2011-10-04 20:49:23 CDTGO:0045860 positive regulation of protein kinase activity (P)PMID:14624253ECO:0000270 expression pattern evidence used in manual assertion

Figure 1, Fig 2BC, Fig 3CE

challenge
unacceptableHUMAN:PTENAfg8920, Team Cheesesteak2011-10-04 20:30:49 CDTGO:0016314 phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity (F)PMID:12622404ECO:0000315 mutant phenotype evidence used in manual assertion

Figure one shows that Akt activity in hippocampal neurons is regulated by PTEN

challenge
requireschangesHUMAN:GRP1Mkline, Team Cheesesteak2011-10-04 20:21:26 CDTGO:0010628 positive regulation of gene expression (P)PMID:21966541ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3

challenge
acceptableHUMAN:KAT2BAfg8920, Team Cheesesteak2011-10-04 20:08:33 CDTGO:0004402 histone acetyltransferase activity (F)PMID:16829519ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 demonstrates that PCAF-mediates acetylation of PTEN lysines 125 and 128.

challenge
acceptableHUMAN:GRP1Mkline, Team Cheesesteak2011-10-04 20:00:46 CDTGO:0043406 positive regulation of MAP kinase activity (P)PMID:15899849ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 4B. Erk1/Erk2 phosphorylation (via MAPK) was delayed and reduced in RasGRP1- mutants.

challenge
acceptableHUMAN:CSK21Afg8920, Team Cheesesteak2011-10-04 19:46:28 CDTGO:0004674 protein serine/threonine kinase activity (F)PMID:11035045ECO:0000315 mutant phenotype evidence used in manual assertion

Figure three shows CK2 phosphorylates the C terminus of PTEN.

challenge
acceptableECOLI:RUVBAfg8920, Team Cheesesteak2011-09-24 22:47:14 CDTGO:0004003 ATP-dependent DNA helicase activity (F)PMID:8433990ECO:0000314 direct assay evidence used in manual assertion

Figure one shows the catalyzed unwinding of the oligonucleotide from the single-stranded circle

challenge
acceptableECOLI:UVRDAfg8920, Team Cheesesteak2011-09-24 21:09:35 CDTGO:0003678 DNA helicase activity (F)PMID:2942537ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows that Helicase II translocates along single-stranded DNA

in the 3' to 5' direction

challenge

Pages in category "Team Cheesesteak"

The following 2 pages are in this category, out of 2 total.


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