PMID:11550008

Citation	Gapin, L, Matsuda, JL, Surh, CD and Kronenberg, M (2001) NKT cells derive from double-positive thymocytes that are positively selected by CD1d. Nat. Immunol. 2:971-8
Abstract	CD1d-reactive NKT cells are a separate T cell sublineage. Instructive models propose that NKT cells branch off the mainstream developmental pathway because of their T cell antigen receptor specificity, whereas stochastic models would propose that they develop from precursor cells committed to this sublineage before variable-gene rearrangement. We show here that immature double-positive (DP) thymocytes form the canonical rearranged Valpha gene of NKT cells at nearly equivalent frequencies in the presence or absence of CD1d expression. After interacting with CD1d in the thymus, these cells give rise to expanded populations of NKT cells-including both CD4+ and double-negative lymphocytes in the thymus and periphery-that express this alpha chain. These results confirm the existence of a DP intermediate for CD1d-reactive NKT cells. They also show that the early developmental stages of these T cells are not governed by a distinct mechanism, which is consistent with the TCR-instructive model of differentiation.
Links	PubMed Online version:10.1038/ni710
Keywords	Animals; Antigens, CD1/analysis; Antigens, CD1/genetics; Antigens, CD1d; CD4-Positive T-Lymphocytes/immunology; CD8-Positive T-Lymphocytes/immunology; Cell Differentiation; Cell Lineage; Cells, Cultured; Galactosylceramides/metabolism; Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; Immunoglobulin Joining Region/biosynthesis; Immunoglobulin Joining Region/genetics; Immunoglobulin Variable Region/biosynthesis; Immunoglobulin Variable Region/genetics; Immunophenotyping; Lymphocyte Activation; Mice; Mice, Knockout; RNA, Messenger/biosynthesis; Stem Cells/cytology; T-Lymphocyte Subsets/classification; T-Lymphocyte Subsets/immunology; Thymus Gland/growth & development; Thymus Gland/immunology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:CD1D1	involved_in	GO:0033084: regulation of immature T cell proliferation in thymus	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:CD1D1		GO:0033084: regulation of immature T cell proliferation in thymus	ECO:0000315:		P		Fig 1c: DPdull tetramers are derived from CD1d-mediated selection; they were no longer present in 9-day old mice thymi	complete CACAO 2867

References

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