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User:Ashayes

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
correctedthroughchallengesMAIZE:UFOG12013-04-14 09:58:18 CDTGO:0047213 anthocyanidin 3-O-glucosyltransferase activity (F)PMID:431836ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows the reaction catalyzed by UFGT (UDPglucose:flavonoid

glucosyltransferase) to be from either flavonols or anthocyanidins to give the respective glucosides.

challenge
updatedbyinstructorARATH:Q9SX142013-04-28 15:24:59 CDTGO:0048438 floral whorl development (P)PMID:2535466ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1B, Figure 2B shows the SEM micrograph of four stages of flower development, with a nested flower (sepals and petals replacing stamens and carpels).

Table 1 summarizes their findings, with the third and fourth whorl of mutant flowers differing from wild-type.

challenge
updatedbyinstructorARATH:AP22013-04-28 16:54:18 CDTGO:0010093 specification of floral organ identity (P)PMID:2535466ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2C shows the SEM micrograph of four stages of flower development, with sepals replaced by leaves.

Table 1 summarizes the findings, with the first and second whorl of mutant flowers differing from wild-type, varying depending on temperature.

challenge
updatedbyinstructorARATH:AP32013-04-28 17:06:02 CDTGO:0010093 specification of floral organ identity (P)PMID:2535466ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2D shows the SEM micrograph of four stages of flower development, with sepals developing in place of petals.

Table 1 summarizes the findings, with the second and third whorl of mutant flowers differing from wild-type, varying depending on temperature. The higher the temperature, the more altered the organs.

challenge
updatedbyinstructorARATH:PIST2013-04-28 17:14:54 CDTGO:0010093 specification of floral organ identity (P)PMID:2535466ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2E shows the SEM micrograph of four stages of flower development, with no third whorl.

Table 1 summarizes their findings, with the second, third, and fourth whorl of mutant flowers differing from wild-type.

challenge
updatedbyinstructorARATH:SUP2013-04-28 17:53:32 CDTGO:0010093 specification of floral organ identity (P)PMID:1352237ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1

challenge
acceptableARATH:Q7X9H32013-05-12 16:37:31 CDTGO:0030308 negative regulation of cell growth (P)PMID:20088901ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 demonstrates the morphological differences in cell size in wild type and mutants. Note that the size of the loss-of-function mutant cells are larger.

Table 2 quantifies the difference in size, but not in number, indicating that GOA downregulates cell expansion, but has minimal/no effect on cell proliferation.

challenge

acceptable:1
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Ashayes

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
correctedthroughchallengesAshayes,
Team Mendel
MAIZE:UFOG1GO:0047213 - anthocyanidin 3-O-glucosyltransferase activity (F)PMID:431836ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

fixed by Ashayes
updatedbyinstructorBewu,
Team Database Backend
LACGS:A0A133PHV7GO:0004565 - beta-galactosidase activity (F)PMID:18603798ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
unacceptableShwei,
Team Database Backend
ARATH:CNX1GO:0005515 - protein binding (F)PMID:23630326ECO:0000021 physical interaction evidencechallengeC: 1
updatedbyinstructorShwei,
Team Database Backend
ARATH:WRK40GO:0045892 - negative regulation of DNA-templated transcription (P)PMID:23509177ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
requireschangesBewu,
Team Database Backend
MOUSE:HIG1AGO:0005515 - protein binding (F)PMID:23646141ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
requireschangesChlxu,
Team quaternary structure
DROME:GRKGO:0051702 - biological process involved in interaction with symbiont (P)PMID:22193955ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

1 annotations fixed by Ashayes

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