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PMID:23509177

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Citation

Van Aken, O, Zhang, B, Law, S, Narsai, R and Whelan, J (2013) AtWRKY40 and AtWRKY63 Modulate the Expression of Stress-Responsive Nuclear Genes Encoding Mitochondrial and Chloroplast Proteins. Plant Physiol. 162:254-71

Abstract

The expression of a variety of nuclear genes encoding mitochondrial proteins is known to adapt to changes in environmental conditions and retrograde signaling. The presence of putative WRKY transcription factor binding sites (W-boxes) in the promoters of many of these genes prompted a screen of 72 annotated WRKY factors in the Arabidopsis (Arabidopsis thaliana) genome for regulators of transcripts encoding mitochondrial proteins. A large-scale yeast one-hybrid screen was used to identify WRKY factors that bind the promoters of marker genes (Alternative oxidase1a, NADH dehydrogenaseB2, and the AAA ATPase Ubiquinol-cytochrome c reductase synthesis1), and interactions were confirmed using electromobility shift assays. Transgenic overexpression and knockout lines for 12 binding WRKY factors were generated and tested for altered expression of the marker genes during normal and stress conditions. AtWRKY40 was found to be a repressor of antimycin A-induced mitochondrial retrograde expression and high-light-induced signaling, while AtWRKY63 was identified as an activator. Genome-wide expression analysis following high-light stress in transgenic lines with perturbed AtWRKY40 and AtWRKY63 function revealed that these factors are involved in regulating stress-responsive genes encoding mitochondrial and chloroplast proteins but have little effect on more constitutively expressed genes encoding organellar proteins. Furthermore, it appears that AtWRKY40 and AtWRKY63 are particularly involved in regulating the expression of genes responding commonly to both mitochondrial and chloroplast dysfunction but not of genes responding to either mitochondrial or chloroplast perturbation. In conclusion, this study establishes the role of WRKY transcription factors in the coordination of stress-responsive genes encoding mitochondrial and chloroplast proteins.

Links

PubMed PMC3641207 Online version:10.1104/pp.113.215996

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:WRK63

GO:0044212: transcription regulatory region DNA binding

ECO:0000314:

F

Figure 2.

complete
CACAO 8465

ARATH:WRK63

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:WRK13

GO:0005515: protein binding

ECO:0000021:

UniProtKB:Q9C983


F

Figure 7C.

complete
CACAO 8463

ARATH:WRK13

GO:0044212: transcription regulatory region DNA binding

ECO:0000314:

F

Figure 2.

complete
CACAO 8468

ARATH:WRK13

GO:0005515: protein binding

ECO:0000021:

UniProtKB:Q9SAH7


F

Figure 7C.

complete
CACAO 8473

ARATH:WRK13

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:WRK57

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:WRK57

GO:0044212: transcription regulatory region DNA binding

ECO:0000314:

F

Figure 2.

complete
CACAO 8464

ARATH:WRK75

GO:0044212: transcription regulatory region DNA binding

ECO:0000314:

F

Figure 2.

complete
CACAO 8466

ARATH:WRK75

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:WRKY9

GO:0044212: transcription regulatory region DNA binding

ECO:0000314:

F

Figure 2.

complete
CACAO 8467

ARATH:WRKY9

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:WRK30

GO:0044212: transcription regulatory region DNA binding

ECO:0000314:

F

Figure 2.

complete
CACAO 8470

ARATH:WRK30

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:WRK42

GO:0044212: transcription regulatory region DNA binding

ECO:0000314:

F

Figure 2.

complete
CACAO 8483

ARATH:WRK42

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:WRK45

GO:0044212: transcription regulatory region DNA binding

ECO:0000314:

F

Figure 2.

complete
CACAO 8484

ARATH:WRK45

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:WRK40

GO:0045892: negative regulation of transcription, DNA-templated

ECO:0000315:

P

Figure 5.

complete
CACAO 8454

ARATH:WRK40

GO:0044212: transcription regulatory region DNA binding

ECO:0000314:

F

Figure 2.

complete
CACAO 8455

ARATH:WRK40

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:WRK40

involved_in

GO:0045892: negative regulation of transcription, DNA-templated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WRK27

GO:0044212: transcription regulatory region DNA binding

ECO:0000314:

F

Figure 2.

complete
CACAO 8469

ARATH:WRK27

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:WRK33

GO:0044212: transcription regulatory region DNA binding

ECO:0000314:

F

Figure 2.

complete
CACAO 8476

ARATH:WRK33

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:WRK15

GO:0044212: transcription regulatory region DNA binding

ECO:0000314:

F

Figure 2.

complete
CACAO 8477

ARATH:WRK15

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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