User:Chlxu

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	BRADI:Q6TCI2	2013-04-12 10:40:28 CDT	GO:0009611 response to wounding (P)	PMID:20565595	ECO:0000314 direct assay evidence used in manual assertion	Fig. 3A	challenge
acceptable	BRADI:Q6TCI2	2013-04-12 10:40:28 CDT	GO:0032260 response to jasmonic acid stimulus involved in jasmonic acid and ethylene-dependent systemic resistance (P)	PMID:20565595	ECO:0000314 direct assay evidence used in manual assertion	Fig. 3C	challenge
requireschanges	DROME:GRK	2013-04-24 22:44:00 CDT	GO:0051702 interaction with symbiont (P)	PMID:22193955	ECO:0000314 direct assay evidence used in manual assertion	Fig.1,Wolbachia titer is affected by the host grk gene in stage 10A oocytes.Fig.2. Host grk dosage affects Wolbachia titer in stage 10A nurse cells.Fig.3.Wolbachia titer is affected by grk at stage 2–3 of oogenesis	challenge
acceptable	9RICK:Q93LF0	2013-04-25 19:56:31 CDT	GO:0006813 potassium ion transport (P)	PMID:12652910	ECO:0000247 sequence alignment evidence used in manual assertion	Figure 2 shows the alignment of e.coli K12 KefB to wolbachia KefKL gene. E.coli K12 KefB is annotated as potassium ion transport inferred from genetic interaction reported in the PMID:3301813.	challenge
updatedbyinstructor	WOLPM:Q73GA4	2013-04-25 20:27:45 CDT	GO:0019153 protein-disulfide reductase (glutathione) activity (F)	PMID:18387819	ECO:0000314 direct assay evidence used in manual assertion	The protein identifier is not present in the paper, but the locus number WD_1055 and whole genome accession number AE017196 are provided in the beginning of the material and method section. Genbank search finds the protein protein_id="AAS14712.1"/note="Identified by similarity to GP:15155781" Fig.5 "showed that a-DsbA1 exhibits oxidoreductase activity in the insu- lin reductase activity, at about the same level of activity as EcDsbA."	challenge
acceptable	WOLPM:Q73GA4	2013-04-25 20:37:44 CDT	GO:0003756 protein disulfide isomerase activity (F)	PMID:18387819	ECO:0000314 direct assay evidence used in manual assertion	The protein identifier is not present in the paper, but the locus number WD_1055 and whole genome accession number AE017196 are provided in the beginning of the material and method section. Genbank search finds the protein protein_id="AAS14712.1"/note="Identified by similarity to GP:15155781" Fig.6 "a-DsbA1 has an activity similar to that of EcDsbA in the ScRNaseA isomerase assay."	challenge
acceptable	WOLTR:Q5GSQ0	2013-04-26 10:21:36 CDT	GO:0044212 transcription regulatory region DNA binding (F)	PMID:23251587	ECO:0000314 direct assay evidence used in manual assertion	Fig.4A "In electrophoretic mobility shift assays (EMSA), FAM-labeled DNA probes derived from the upstream region of ribA, virB8-1, virB3, virB4-2, virB8-2, virB9-2, sodA, wBmxR1 and wBmxR2, and were incubated with (+) or without (–) protein and loaded onto a 6% DNA retardation gel." "(EMSA) showed wBmxR1 binds to regions upstream of the virB9-2, ribA, sodA and wBmxR2 genes (Fig. 4A, C)"	challenge
acceptable	WOLTR:Q5GSD8	2013-04-26 10:27:51 CDT	GO:0044212 transcription regulatory region DNA binding (F)	PMID:23251587	ECO:0000314 direct assay evidence used in manual assertion	Fig.4B "In electrophoretic mobility shift assays (EMSA), FAM-labeled DNA probes derived from the upstream region of ribA, virB8-1, virB3, virB4-2, virB8-2, virB9-2, sodA, wBmxR1 and wBmxR2, and were incubated with (+) or without (–) protein and loaded onto a 6% DNA retardation gel. " "(EMSA) showed ... wBmxR2 binds to virB4-2, ribA, and the wBmxR1 promoter regions (Fig. 4B, C)"	challenge
unacceptable	WOLSP:FTSZ	2013-05-06 12:19:30 CDT	GO:0051301 cell division (P)	PMID:7689140	ECO:0000247 sequence alignment evidence used in manual assertion	Fig 3. In E.coli K12, ftsZ functions in cell division inferred from mutant phenotype PubMed 2045370. Source: EcoliWiki	challenge
unacceptable	WOLSP:FTSZ	2013-05-06 12:19:30 CDT	GO:0005737 cytoplasm (C)	PMID:7689140	ECO:0000247 sequence alignment evidence used in manual assertion	Fig 3. In E.coli K12, ftsZ is located in cytoplasm inferred from direct assay PubMed 1943703. Source: EcoliWiki	challenge
acceptable	WOLSP:FTSZ	2013-05-06 12:19:30 CDT	GO:0005525 GTP binding (F)	PMID:7689140	ECO:0000247 sequence alignment evidence used in manual assertion	Fig 3. In E.coli K12, ftsZ has GTP binding activity inferred from direct assay PMID:1528267 PMID:1528268 . Source: EcoliWiki	challenge
unacceptable	WOLSP:FTSZ	2013-05-06 12:19:31 CDT	GO:0051258 protein polymerization (P)	PMID:7689140	ECO:0000247 sequence alignment evidence used in manual assertion	Fig 3. In E.coli K12, ftsZ functions in protein polymerization inferred from direct assay PubMed 21321206 and PubMed 8917533 Source: EcoliWiki	challenge
acceptable	ARATH:VOZ1	2013-05-10 10:45:08 CDT	GO:0009631 cold acclimation (P)	PMID:23167462	ECO:0000316 genetic interaction evidence used in manual assertion	Fig 1. direct assay of double mutant cold resistance Fig 3. change in cold-resistance gene expression in double mutant	challenge
updatedbyinstructor	ARATH:VOZ1	2013-05-10 10:58:03 CDT	GO:0009414 response to water deprivation (P)	PMID:23167462	ECO:0000316 genetic interaction evidence used in manual assertion	Fig 5. Drought resistance is increased in VOZ1-VOZ2 double mutant	challenge
acceptable	ARATH:VOZ1	2013-05-10 11:42:56 CDT	GO:0009408 response to heat (P)	PMID:23167462	ECO:0000316 genetic interaction evidence used in manual assertion	Fig 6. Heat tolerance is decreased in VOZ1-VOZ2 double mutant, and VOZ2 expression level change in response to heat.	challenge
acceptable	ARATH:VOZ1	2013-05-10 11:55:12 CDT	GO:0042742 defense response to bacterium (P)	PMID:23167462	ECO:0000316 genetic interaction evidence used in manual assertion	Fig 8. Double mutant shows higher susceptibility to Pseudomonas syringae needs relation RO:0002264 "acts upstream or within"	challenge
updatedbyinstructor	HUMAN:T2R38	2013-05-12 11:57:04 CDT	GO:0005886 plasma membrane (C)	PMID:12379855	ECO:0000314 direct assay evidence used in manual assertion	Fig.2 a	challenge
updatedbyinstructor	ARATH:SGGP	2013-05-12 12:18:23 CDT	GO:0050308 sugar-phosphatase activity (F)	PMID:23179445	ECO:0000314 direct assay evidence used in manual assertion	Fig 3.	challenge

acceptable:10
unacceptable:3
requires_changes:1
flagged:0

Annotations challenged by Chlxu

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Monica Yun, Team Beadle	MAIZE:EMB5	GO:0003677 - DNA binding (F)	PMID:23559745	ECO:0000255 match to sequence model evidence used in manual assertion	challenge	C: 1
requireschanges	Michael B Chun, Team Tree	?	GO:0051879 - Hsp90 protein binding (Fig. 1E shows that both HvSGT1 (SGT1 from barley) and AtSGT1b (SGT1b from Arabidopsis) interact with full-length HvHSP90 (HSP90 from barley); the HSP90-binding function is likely conserved between monocots and dicots.)	PMID:14504384	IDA: Inferred from Direct Assay	challenge	C: 2
updatedbyinstructor	Mmvilla, Team G'nUnit	BACSU:FTSZ	GO:0003924 - GTPase activity (F)	PMID:23577149	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1 fixed by Chlxu
updatedbyinstructor	Mmvilla, Team G'nUnit	BACSU:MINC	GO:0032272 - negative regulation of protein polymerization (P)	PMID:23577149	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2 fixed by Chlxu

2 annotations fixed by Chlxu

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