PMID:23167462

Citation	Nakai, Y, Nakahira, Y, Sumida, H, Takebayashi, K, Nagasawa, Y, Yamasaki, K, Akiyama, M, Ohme-Takagi, M, Fujiwara, S, Shiina, T, Mitsuda, N, Fukusaki, E, Kubo, Y and Sato, MH (2013) Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis. Plant J. 73:761-75
Abstract	Plants adapt to abiotic and biotic stresses by activating abscisic acid-mediated (ABA) abiotic stress-responsive and salicylic acid-(SA) or jasmonic acid-mediated (JA) biotic stress-responsive pathways, respectively. Although the abiotic stress-responsive pathway interacts antagonistically with the biotic stress-responsive pathways, the mechanisms that regulate these pathways remain largely unknown. In this study, we provide insight into the function of vascular plant one-zinc-finger proteins (VOZs) that modulate various stress responses in Arabidopsis. The expression of many stress-responsive genes was changed in the voz1voz2 double mutant under normal growth conditions. Consistent with altered stress-responsive gene expression, freezing- and drought-stress tolerances were increased in the voz1voz2 double mutant. In contrast, resistance to a fungal pathogen, Colletotrichum higginsianum, and to a bacterial pathogen, Pseudomonas syringae, was severely impaired. Thus, impairing VOZ function simultaneously conferred increased abiotic tolerance and biotic stress susceptibility. In a chilling stress condition, both the VOZ1 and VOZ2 mRNA expression levels and the VOZ2 protein level gradually decreased. VOZ2 degradation during cold exposure was completely inhibited by the addition of the 26S proteasome inhibitor, MG132, a finding that suggested that VOZ2 degradation is dependent on the ubiquitin/26S proteasome system. In voz1voz2, ABA-inducible transcription factor CBF4 expression was enhanced significantly even under normal growth conditions, despite an unchanged endogenous ABA content. A finding that suggested that VOZs negatively affect CBF4 expression in an ABA-independent manner. These results suggest that VOZs function as both negative and positive regulators of the abiotic and biotic stress-responsive pathways, and control Arabidopsis adaptation to various stress conditions.
Links	PubMed Online version:10.1111/tpj.12069
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:VOZ1		GO:0009408: response to heat	ECO:0000316:	UniProtKB:Q9SLB9	P		Fig 6. Heat tolerance is decreased in VOZ1-VOZ2 double mutant, and VOZ2 expression level change in response to heat.	complete CACAO 8243
ARATH:VOZ1		GO:0042742: defense response to bacterium	ECO:0000316: genetic interaction evidence used in manual assertion	UniProtKB:Q9SLB9	P		Fig 8. Double mutant shows higher susceptibility to Pseudomonas syringae needs relation RO:0002264 "acts upstream or within"	complete CACAO 8244
ARATH:VOZ1		GO:0009631: cold acclimation	ECO:0000316:	UniProtKB:Q9SLB9	P		Fig 1. direct assay of double mutant cold resistance Fig 3. change in cold-resistance gene expression in double mutant	complete CACAO 8240
ARATH:VOZ1		GO:0009414: response to water deprivation	ECO:0000316:	UniProtKB:Q9SLB9	P		Fig 5. Drought resistance is increased in VOZ1-VOZ2 double mutant	complete CACAO 8241
ARATH:VOZ1	involved_in	GO:0009414: response to water deprivation	ECO:0000316: genetic interaction evidence used in manual assertion	UniProtKB:Q9SLB9	P		Seeded From UniProt	complete
ARATH:VOZ1	involved_in	GO:0009408: response to heat	ECO:0000316: genetic interaction evidence used in manual assertion	UniProtKB:Q9SLB9	P		Seeded From UniProt	complete

References

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